Multiplex immunoline test for food allergens detection. Poster a sborník 15th International Nutrition and Diagnostics Conference (INDC 2015) Prague, Czech Republic, October 5 - 8, 2015, ISBN978-80-7395-901-2

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027022%3A_____%2F15%3A%230000030" target="_blank" >RIV/00027022:_____/15:#0000030 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Multiplex immunoline test for food allergens detection. Poster a sborník 15th International Nutrition and Diagnostics Conference (INDC 2015) Prague, Czech Republic, October 5 - 8, 2015, ISBN978-80-7395-901-2

Original language description

Some allergenic products and substances contained in food must be indicated on the label in accordance with the EU legislation (Regulation EU No 1169/2011). Cereals containing gluten, milk, eggs, nuts, peanuts, soybeans, fish, crustaceans, molluscs, celery, lupin, sesame, mustard and sulphites belong to the group of mandatory declared allergens. The development of a highly sensitive and specific multiplex test able to detect more allergens within a single assay was aim of this work. Multiplex Immunoline test is specific against allergens Arah1 (peanut), beta-lactoglobulin (milk), casein (milk), egg white proteins (egg), tree nuts proteins from hazelnuts, walnuts, almonds, pistachios, Brazil nuts, macadamia nuts and cashew and mustard proteins from Sinapsis alba, Brassica nigra, Brassica juncea, Rape seed. The presence of food allergens can be find out within a short time, which can lead to time savings for food producers and trade in food commodities. Multiplex Immunoline test is based on the binding of antibodies immobilized on the solid phase to an antigens extracted from food samples. The detection is carried out by the biotinylated specific antibodies, streptavidin - alkaline phosphatase and a BCIP/NBT colorimetrics membrane AP substrate. The different categories of commercial food products were tested for the Immunoline validation and the stability and reproducibility tests were performed.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

GM - Food industry

OECD FORD branch

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Project

<a href="/en/project/TA03010625" target="_blank" >TA03010625: New materials and technologies for development of multiplex test for complex diagnostics in medicine and food allergens detection</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů