International interlaboratory study on TaqMan real-time polymerase chain reaction authentication of black seabream (Spondyliosoma cantharus)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027022%3A_____%2F18%3AN0000008" target="_blank" >RIV/00027022:_____/18:N0000008 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/18:10390775 RIV/60461373:22330/18:43917524 RIV/75010330:_____/18:00012196

Result on the web

<a href="http://www.vup.sk/en/index.php?mainID=2&navID=34&version=2&volume=57&article=2084" target="_blank" >http://www.vup.sk/en/index.php?mainID=2&navID=34&version=2&volume=57&article=2084</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

International interlaboratory study on TaqMan real-time polymerase chain reaction authentication of black seabream (Spondyliosoma cantharus)

Original language description

An interlaboratory study of five laboratories testing real-time polymerase chain reaction (PCR) with TaqMan probe detection of black seabream (Spondyliosoma cantharus) fish is presented in this work. The method is oriented to the intron of the nuclear gene encoding the main protein of the musculature of the fish, parvalbumin. This feature distinguishes the employed approach from those based on mitochondrial genes. Here, the intron is flanked by exon stretches highly conserved among species. This provides a unique advantage when a new fish species emerges as a commodity on the market: Species-versatile degenerate primers can be easily designed on these conserved exon stretches. Therefore, the initial uncertainty of the species-specific sequence of the intron can, in such case, be bypassed during the adoption of the method to this particular new species, because the amplicon obtained at this pilot stage provides the sequence of the intron itself. DNA isolates from eight exemplars of S. cantharus and from nineteen other fish species, the latter being used as negative controls, were tested in this study by participating laboratories. The readouts in qualitative assessment were 100% accurate. The quantitative results provided an average value and variation among samples representing particular exemplars of S. cantharus.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40103 - Fishery

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Food and Nutrition Research

ISSN

1336-8672

e-ISSN

1338-4260

Volume of the periodical

57

Issue of the periodical within the volume

1

Country of publishing house

SK - SLOVAKIA

Number of pages

11

Pages from-to

27-37

UT code for WoS article

000430630000003

EID of the result in the Scopus database

2-s2.0-85044600650