Somatic embryogenesis in Phlox paniculata - histological analysis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027073%3A_____%2F16%3AN0000008" target="_blank" >RIV/00027073:_____/16:N0000008 - isvavai.cz</a>

Result on the web

<a href="https://www.degruyter.com/view/j/biolog.2016.71.issue-7/biolog-2016-0100/biolog-2016-0100.xml" target="_blank" >https://www.degruyter.com/view/j/biolog.2016.71.issue-7/biolog-2016-0100/biolog-2016-0100.xml</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1515/biolog-2016-0100" target="_blank" >10.1515/biolog-2016-0100</a>

Result language

angličtina

Original language name

Somatic embryogenesis in Phlox paniculata - histological analysis

Original language description

In vitro regeneration and histological analysis of somatic embryogenesis derived from in vitro leaves were assessed in Phlox paniculata L. The explants were incubated on Murashige and Skoog medium containing 2.26-4.52 mu M 2,4-dichlorophenoxyacetic acid (2,4-D) with or without 0.89 mu M benzyladenine supplemented with 3% or 9% sucrose and vitamins. The intact leaf blades were cultured in the dark at temperature 22 +/- 2 degrees C. Two Phlox cultivars showed significantly higher regeneration capacity in the in vitro conditions when the cultivation media have been supplemented with 2,4-D at concentrations of 4.52 mu M and 3% sucrose. The effect of 2,4-D on somatic embryo induction has been enhanced in presence of cytokinin benzyladenine. In cultivars, the embryogenic structures have been created on calluses derived from leaf sections within 6-9 weeks in culture. Meristematic zones with forming embryogenic structures were mostly localized adjacent to de novo forming tracheal elements in calluses in our experiments. In the absence of tracheal elements, the embryogenic structures have been surrounded by several layers of isodiametric cells. The embryo proper and the suspensor part could be clearly distinguished on the embryogenic structures. The embryogenic potential of cultures has been very high, so that even secondary and tertiary somatic embryos were formed. Within 6-9 weeks in culture, well differentiated embryogenic structures up to torpedo stages were developed. Cultivar 'Fuji' exhibited slightly higher embryogenic response in culture than cultivar 'Starfire'. The cultures maintained their embryogenic potential for about 18 months. Embryogenic structures have been able to convert to complete plants within 3 months.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EI - Biotechnology and bionics

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biologia

ISSN

0006-3088

e-ISSN

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Volume of the periodical

71

Issue of the periodical within the volume

7

Country of publishing house

DE - GERMANY

Number of pages

6

Pages from-to

763-768

UT code for WoS article

000382261100006

EID of the result in the Scopus database

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