Initiation and histology of somatic embryogenesis and organogenesis in Phlox paniculata L.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F07%3A23442" target="_blank" >RIV/60460709:41210/07:23442 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Initiation and histology of somatic embryogenesis and organogenesis in Phlox paniculata L.

Original language description

Histological analysis of somatic embryogenesis and organogenesis derived from in vitro-grown leaves were assessed in Phlox paniculata L. Phlox cultivars ´Fuji´ and ´Starfire´ belong to important border perennials and are different in anthocyan occurrence. The leaf explants from sterile cultures were incubated on full-strenght MS salts (Murashige and Skoog, 1962) medium containing 0.5?1.0 mg.l-1 2,4-D (2,4-dichlorophenoxyacetic acid) with/without 0.2 mg.l-1 BA (benzyladenine). MS medium was supplementedwith 30 or 90 g l-1 sucrose, B5 vitamins and 7.5 g l-1 agar Sigma. The intact leaf blades were cultured (abaxial side down) in the dark at temperature 22 +- 2 °C. The regeneration rate was expressed as the percentage of explant total number and the structures formed. The regeneration was highly depended on the genotype and concentration of 2,4-D and BA present in the culture medium. The significantly highest rate of regeneration (about 90 %) and callus induction were obtained on medium w

Czech name

Iniciace a histologie somatické embryogeneze a organogeneze u Phlox paniculata L.

Czech description

Histological analysis of somatic embryogenesis and organogenesis derived from in vitro-grown leaves were assessed in Phlox paniculata L. Phlox cultivars ´Fuji´ and ´Starfire´ belong to important border perennials and are different in anthocyan occurrence. The leaf explants from sterile cultures were incubated on full-strenght MS salts (Murashige and Skoog, 1962) medium containing 0.5?1.0 mg.l-1 2,4-D (2,4-dichlorophenoxyacetic acid) with/without 0.2 mg.l-1 BA (benzyladenine). MS medium was supplementedwith 30 or 90 g l-1 sucrose, B5 vitamins and 7.5 g l-1 agar Sigma. The intact leaf blades were cultured (abaxial side down) in the dark at temperature 22 +- 2 °C. The regeneration rate was expressed as the percentage of explant total number and the structures formed. The regeneration was highly depended on the genotype and concentration of 2,4-D and BA present in the culture medium. The significantly highest rate of regeneration (about 90 %) and callus induction were obtained on medium w

Type

D - Article in proceedings

CEP classification

ED - Physiology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Propagation of Ornamental Plants. Proc. of the 5th Intern. Conference ?Propagation of Ornamental Plants

ISBN

978-954-90425-4-2

ISSN

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e-ISSN

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Number of pages

1

Pages from-to

70-70

Publisher name

SEJANI Publisher

Place of publication

Sofia, Bulgaria

Event location

Sofia, Bulgaria

Event date

Sep 5, 2007

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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