Production of scFv recombinant fragments against 2,4-dichlorophenoxyacetis acid hapten using naive phage library

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F03%3A00008470" target="_blank" >RIV/00027162:_____/03:00008470 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Production of scFv recombinant fragments against 2,4-dichlorophenoxyacetis acid hapten using naive phage library

Original language description

Three single chain variable fragment (scFv) antibodies against 2,4-dichlophenoxyacetic acid (2,4-D) herbicide were produced by the Griffin1.library. The selection of the scFv from the phage library was carried out by 2,4-D-protein coated tubes with different levels of hapten substitution in the conjugate. The scFv phage clones were isolated within the five round library panning and the antibodies were expressed in Escherichia coli HB2151. The recombinant products were purified by metal affinity chromatography yielding 200 ?g of pure scFv per 1 liter of bacterial culture. The antibody fragments provided steep curves in conventional indirect ELISA having the IC50 values from 10.2 to 14.5 ng/ml established for 2,4-D standard. Interestingly enough, the recombinant ScFv E1 anti-body exhibited 68% cross-reactivity with 2,4-dichlorphenol (2,4-D = 100%), and 38.0% with methylchlorophenoxy-acetic acid (MCPA) whereas reaction with other phenoxyacetic compounds was low. Similar characteristics we

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EI - Biotechnology and bionics

OECD FORD branch

—

Project

<a href="/en/project/GA525%2F00%2F1261" target="_blank" >GA525/00/1261: Production of recombinant antibodies for environmental and food analysis</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Veterinární medicína

ISSN

0375-8427

e-ISSN

—

Volume of the periodical

48

Issue of the periodical within the volume

9

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

11

Pages from-to

237-247

UT code for WoS article

—

EID of the result in the Scopus database

—