In vitro neutralization of Equid herpesvirus 1 mediated by recombinant antibodies

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F08%3A00026655" target="_blank" >RIV/00216224:14310/08:00026655 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16170/08:00001819
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
In vitro neutralization of Equid herpesvirus 1 mediated by recombinant antibodies
Original language description
A phage antibody display library of single chain fragment variables (scFv) was applied to develop anti-equid herpesvirus-1 (EHV-1) glycoprotein D (gD) neutralizing antibodies. To enrich for specific scFvs, the phage antibody library was panned against epitope derived from the N-terminal part of EHV-1 gD. Unique clones were differentiated by BstNI fingerprinting and further characterized by sequencing and immunoreactivity. The neutralizing effect of each clone was assessed by plaque reduction assay. Three clones with neutralizing effect were isolated.
Czech name
In vitro neutralizace Equid herpesviru 1 zprostředkovaná rekombinantními protilátkami
Czech description
Pomocí fágové knihovny byly připraveny scFv protilátky schopné neutralizovat EHV-1 virus.

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
<a href="/en/project/GP524%2F06%2FP051" target="_blank" >GP524/06/P051: The selection and use of recombinant antibodies against glycoprotein G type specific epitopes of equine herpesviruses 1 and 4</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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