Intestinal colonisation-inhibition and virulence of Salmonella phoP, rpoS and ompC deletion mutants in chickens

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F04%3A%230000146" target="_blank" >RIV/00027162:_____/04:#0000146 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Intestinal colonisation-inhibition and virulence of Salmonella phoP, rpoS and ompC deletion mutants in chickens

Original language description

In this study, mutants of Salmonella Typhimurium and Enteritidis with deletions in phoP and rpoS, either alone or in combination with ompC, were tested for their level of attenuation and their ability to inhibit the intestinal colonisation of the isogenic parent strains in chickens. Mutants with deletions only in rpoS demonstrated an unaffected potential to inhibit the intestinal colonisation of the challenge strain but were still fully virulent for the chickens. Mutants with deletions in phoP, either alone or in combination with rpoS, resulted in a high level of attenuation, unimpaired ability to colonise the gut and a nearly unaffected potential to inhibit the challenge strain from caecal colonisation. Mutants with an additional deletion in ompC revealed a reduced capacity of intestinal colonisation-inhibition when compared to the control strains and both the single rpoS and the phoP deletion mutants.

Czech name

Inhibice růstu a virulence mutantů salmonel v genech phoP, rpoS a ompC pro kuřata

Czech description

V této práci byli připraveni mutanti Salmonella Typhimurium a Enteritidis s delecemi v genech phoP a rpoS, buď samostatně anebo v kombinaci s delecí genu ompC. Tyto kmeny byly testovány na míru jejich atenuace a schopnost inhibice růstu izogenního divokého kmene při experimentální infekci kuřat. Mutant s delecí pouze v genu rpoS inhiboval růst divokého kmene ale jeho virulence nebyla výrazně snížena. Mutanti s delecí v genu phoP, buď samostatně nebo v kombinaci s rpoS, měli výrazně sníženou virulenci azachovanou schopnost kolonizovat střevní trakt a inhibovat růst divokého kmene. Mutanti s dodatečnou mutací v genu ompC měli sníženou schopnost kolonizovat střevní trakt a rovněž i sníženou schopnost inhibovat růst divokého kmene.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

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Continuities

R - Projekt Ramcoveho programu EK

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Veterinary Microbiology

ISSN

0378-1135

e-ISSN

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Volume of the periodical

98

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

37-43

UT code for WoS article

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EID of the result in the Scopus database

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