A PCR based method for the identification of genetically modified potatoes carrying the gene ac2 from Amaranthus caudatus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F05%3A06000047" target="_blank" >RIV/00027162:_____/05:06000047 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

čeština

Original language name

Využití metody PCR pro identifikaci geneticky modifikovaných brambor nesoucích gen ac2 z Amaranthus caudatus

Original language description

In European Union products consisting of or containing genetically modified organisms (GMO) and food products obtained from GMOs are subject to traceability and labelling requirements.The work is based on developing a system for the detection of anantimicrobial peptide (Ac AMP2) from amaranth (Amaranthus caudatus) which was used for the transformation of potatoes (Solanum tuberosum). The base of the method is duplex PCR which enables identification of both amaranth (ac2) and potato (StTS1)specific genesin one tube. The method is enhanced by using positive plasmid controls which are able to reveal presence of PCR inhibitors and reduce the incidence of false negative results. The analytical method was used for analyse 108 samples ofpotatoes, but none ofthem was positive for genetic modification "ac2".

Czech name

Využití metody PCR pro identifikaci geneticky modifikovaných brambor nesoucích gen ac2 z Amaranthus caudatus

Czech description

In European Union products consisting of or containing genetically modified organisms (GMO) and food products obtained from GMOs are subject to traceability and labelling requirements.The work is based on developing a system for the detection of anantimicrobial peptide (Ac AMP2) from amaranth (Amaranthus caudatus) which was used for the transformation of potatoes (Solanum tuberosum). The base of the method is duplex PCR which enables identification of both amaranth (ac2) and potato (StTS1)specific genesin one tube. The method is enhanced by using positive plasmid controls which are able to reveal presence of PCR inhibitors and reduce the incidence of false negative results. The analytical method was used for analyse 108 samples ofpotatoes, but none ofthem was positive for genetic modification "ac2".

Type

D - Article in proceedings

CEP classification

GM - Food industry

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

VII. konference mladých vědeckých pracovníků

ISBN

80-7305-527-9

ISSN

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e-ISSN

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Number of pages

4

Pages from-to

53-56

Publisher name

Veterinární a farmaceutická univerzita Brno

Place of publication

Brno

Event location

Brno

Event date

Jun 2, 2005

Type of event by nationality

CST - Celostátní akce

UT code for WoS article

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