Embryos produced in vitro from bulls carrying 16;20 and 1;29 Robersonian translocations: detection of translocations in embryos by fluorescence in situ hybridization

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F05%3A06N00402" target="_blank" >RIV/00027162:_____/05:06N00402 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Embryos produced in vitro from bulls carrying 16;20 and 1;29 Robersonian translocations: detection of translocations in embryos by fluorescence in situ hybridization

Original language description

Robertsonian translocation rob(16;20) in the heterozygous state was discovered in a subfertile bull of the Czech Siemmental breed. The present experiments were designed to detect the rob(16;20) and determine its incidence for in vitro produced embryos(the 6- to 8-cell stage and the blastocyst stage), using fluorescence in situ hybridization (FISH) and the rob(1;29) as a detection control. To characterize semen of both bulls with the rob translocations, their sperm was examined for DNA integrity bythe Sperm Chromatin Structure Assay (SCSA). The embryos were fixed in methanol and acetic acid with Tween 20. Painting probes for chromosomes 16 (sp. Green) and 20 (sp. Orange) and chromosomes 1 (sp. Orange) and 29 (sp. Green) were simultaneouslyhybridized. Inthe embryos derived from the rob(16;20) bull, the presence of this translocation was not detected. On the other hand, 52.5 % of the embryos derived from the rob(1;29) bull were translocation carriers.

Czech name

Produkce embryí in vitro od býků nositelů robertsonovské translokace 16;20 a 1;29: detekce translokace v embryích pomocí fluorescenční in situ hybridizace

Czech description

Robertsonovská translokace byla poprvé popsána u subfertilního býka českého simmentálského skotu. Práce si klade za cíl zjistit incidenci této translokace v in vitro produkovaných embryích (ve stadiu 6- 8 buněk a stadiu blastocysty) pomocí fluorescenčníin situ hybridizace (FISH). Jako kontrola je použita translokace rob(1;29). Mimo jiné byla provedena charakteristika integrity chromatinu ve spermiích těchto býků pomocí Sperm Chromatin Structure Assay (SCSA). Embrya byla fixována pomocí metanolu a kys.octové s přídavkem Tweenu 20. Byly použity malovací sondy pro chromosomy 16 (sp. Green) a 20 (sp. Orange), dále pro chromosomy 1 (sp. Orange) a 29 (sp. Green). V embryích pocházejících od býka s rob(16;20) nebyla nalezena fúze v žádném z embryí, zatímco52,5 % embryí pocházející od býka s rob (1;29) neslo translokaci.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/QC1197" target="_blank" >QC1197: Application of molecular method for improving the hereditary health checks in farm animals</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Zygote

ISSN

0967-1994

e-ISSN

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Volume of the periodical

13

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

4

Pages from-to

31-34

UT code for WoS article

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EID of the result in the Scopus database

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