Application of DNA-based Techniques for Intraspecies Differentiation of Hake Fish

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F09%3A%230000552" target="_blank" >RIV/00027162:_____/09:#0000552 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Application of DNA-based Techniques for Intraspecies Differentiation of Hake Fish
Original language description
The aim of this study was to determine hake species distribution in the Czech market by the PCR-RFLP and sequencing of mt cyt b gene and to develop and optimise an alternative system for the determination of hake species by sequencing and/or PCR-RFLP using Pantophysin I sequence. Among 20 samples of hakes obtained in the Czech market three species were identified: North Pacific hake (Merluccius productus), Argentine hake (Merluccius hubbsi) and South Pacific hake (Merluccius gayi). The approaches testedin our study represent a significant tool either for the differentiation of hake species from other gadoid species (PCR) or for intraspecies identification of different hake species (PCR-RFLP, sequencing). This can be applied in fish species substitution detection within the context of consumers' rights protection.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GM - Food industry
OECD FORD branch
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Project
<a href="/en/project/QG60090" target="_blank" >QG60090: Alternative methods for monitoring of the food authenticity</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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