Direct high-performance liquid chromatography analysis of lyophilised liposomal entrapped alpha-tocopheryl succinate

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F10%3A%230000657" target="_blank" >RIV/00027162:_____/10:#0000657 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Direct high-performance liquid chromatography analysis of lyophilised liposomal entrapped alpha-tocopheryl succinate

Original language description

In this study, we develop the HPLC assay for the direct determination of alpha-tocopheryl succinate (TOS) entrapped in the liposomes as well as monitoring of TOS chemical stability in the liposomal lyophilisates during 6-month storage period. The composition of mobile phase was optimised for separation of TOS, alpha-tocopherol (TOH) as TOS degradation product and alpha-tocopheryl acetate as internal standard. The quantification of TOS in liposomal formulation and liposomal sediment enables to calculateentrappment efficiency of TOS (EETOS). For the liposomal lyophilisates stored during 6 months, the obtained values of EETOS were higher than 90%. The chemical stability of liposomal entrapped TOS was investigated by monitoring the level of TOH. Generally, the content of liposomal TOH did not exceed 5% of the liposomal TOS content.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FR - Pharmacology and apothecary chemistry

OECD FORD branch

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Project

<a href="/en/project/KAN200520703" target="_blank" >KAN200520703: The use of ultrasound in nanomedicine</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemické listy

ISSN

0009-2770

e-ISSN

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Volume of the periodical

104

Issue of the periodical within the volume

Supll. 16

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

3

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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