Genotoxic polycyclic aromatic hydrocarbons fail to induce the p53-dependent DNA damage response, apoptosis or cell-cycle arrest in human prostate carcinoma LNCaP cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F10%3A%230000687" target="_blank" >RIV/00027162:_____/10:#0000687 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/10:00347299 RIV/68378041:_____/10:00347299

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Genotoxic polycyclic aromatic hydrocarbons fail to induce the p53-dependent DNA damage response, apoptosis or cell-cycle arrest in human prostate carcinoma LNCaP cells

Original language description

Exposure to polycyclic aromatic hydrocarbons has been positively associated with prostate cancer, but knowledge of the formation of PAH-DNA adducts and related genotoxic events in prostatic cells is limited. In the present study, benzo[a]pyrene, formed significant levels of DNA adducts in cell lines derived from human prostate carcinoma. Despite a significant amount of DNA adducts being formed, neither apoptosis nor cell-cycle arrest were induced in LNCaP cells, presumably due to their failure to activate the p53-dependent DNA damage response. LNCaP cells were not sensitized to the induction of apoptosis by PAHs even through inhibition of the PI3K/Akt pro-survival pathway and the lack of apoptosis was not due a disruption of expression of pro-apoptoticand pro-survival members of the Bcl-2 family of apoptosis regulators. In contrast to other genotoxic stimuli, genotoxic PAHs failed to induce DNA double-strand breaks, as illustrated by the lack of phosphorylation of histone H2AX or Chk-

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

DN - Environmental impact on health

OECD FORD branch

—

Project

<a href="/en/project/GA310%2F07%2F0961" target="_blank" >GA310/07/0961: The role of environmental pollutants in mechanisms regulating development of prostate carcinoma</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

TOXICOLOGY LETTERS

ISSN

0378-4274

e-ISSN

—

Volume of the periodical

197

Issue of the periodical within the volume

3

Country of publishing house

IE - IRELAND

Number of pages

9

Pages from-to

—

UT code for WoS article

000281002700011

EID of the result in the Scopus database

—