Toxic Effects of Methylated Benzo[a]pyrenes in Rat Liver Stem-Like Cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F11%3A%230000808" target="_blank" >RIV/00027162:_____/11:#0000808 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/11:00365862 RIV/68378041:_____/11:00365862
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Toxic Effects of Methylated Benzo[a]pyrenes in Rat Liver Stem-Like Cells
Original language description
The methylated benzo[a]pyrenes (MeBaPs) are present at significant levels in the environment. However, the existing data on their toxic effects in vitro and/or in vivo are still largely incomplete. Transcription factor AhR plays a key role in the metabolic activation of PAHs to genotoxic metabolites, but the AhR activation may also contribute to the tumor promoting effects of PAHs. In this study, the AhR-mediated activity of five selected MeBaP isomers was estimated in the DR-CALUX reporter gene assay performed in rat hepatoma cells. Detection of other effects, including induction of CYP1A1, CYP1B1, and AKR1C9 mRNAs, DNA adduct formation, production of reactive oxygen species, oxidation of deoxyguanosine, and cell cycle modulation and apoptosis, was performed in the rat liver epithelial WB-F344 cell line. We identified 1-MeBaP as the most potent inducer of AhR activation, stable DNA adduct formation, checkpoint kinase 1 and p53 phosphorylation, and apoptosis. These effects suggest that
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
DN - Environmental impact on health
OECD FORD branch
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Project
<a href="/en/project/GA525%2F08%2F1590" target="_blank" >GA525/08/1590: Identification of endocrine disrupters, genotoxins and tumor promoters in abiotic matrixes of urban and agricultural environment</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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