Molecular characterization of a porcine sapovirus strain isolated from a piglet with diarrhoea: a case report

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F11%3A%230000770" target="_blank" >RIV/00027162:_____/11:#0000770 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Molecular characterization of a porcine sapovirus strain isolated from a piglet with diarrhoea: a case report

Original language description

Porcine sapoviruse have been considered as an aethiological agent of gastroenteritis in pigs. In this study, we therefore analysed 251 faecal samples obtained from diarrhoeic pigs in the Czech Republic during January 2005-June 2010 and tested them by negative staining electron microscopy for the presence of sapoviruses. Only one sample showed the presence of viral particles with characteristic sapovirus morphology. Presence of sapovirus was confirmed by RT-PCR assay with primers specific to sapoviral RNA polymerase gene and capsid gene. The phylogenetic analysis based on partial RNA polymerase sequence placed the new Czech isolate into the GVII genogroup of porcine sapoviruses, however, analysis of partial capsid gene sequence classified isolate as GIII of the genus Sapovirus. The findings of phylogenetic analysis indicate, that recombinant porcine sapovirus was identified. According to our knowledge this is the first description of porcine sapovirus in domestic pigs in the Czech Repub

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GJ - Diseases and animal vermin, veterinary medicine

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Veterinární Medicína

ISSN

0375-8427

e-ISSN

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Volume of the periodical

56

Issue of the periodical within the volume

8

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

7

Pages from-to

409-415

UT code for WoS article

000295252600007

EID of the result in the Scopus database

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