Preparation of Metallochelating Microbubbles and Study on Their Site-Specific Interaction with rGFP-HisTag as a Model Protein
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F11%3A%230000773" target="_blank" >RIV/00027162:_____/11:#0000773 - isvavai.cz</a>
Alternative codes found
RIV/67985823:_____/11:00370417
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Preparation of Metallochelating Microbubbles and Study on Their Site-Specific Interaction with rGFP-HisTag as a Model Protein
Original language description
Construction of surface-modi?ed metallochelating microbubbles (MBs) was proved using recombinant green ?uorescent protein (rGFP) with 6 HisTags. MBs composed of DPPC with various contents of metallochelating lipid DOGS-NTA-Ni were prepared by intensive shaking of liposome suspension under atmosphere of sulfur hexa?uoride by 3M ESPE CapMix device. Static light scattering, ?ow cytometry and optical microscopy were used to determine mean size (2.1-2.8 um) and concentration of MBs (109/mL). MBs were lyophilized in the presence of sucrose. These MBs were stable in vitro (half-live of MBs in bovine serum at 37 °C was 3-7 min) and in vivo (mouse model). DPPC-based metallochelating MBs provided clear and very contrast image of the ventricular cavity in mouse heart soon after injection as observed by ultrasonic system. Commercial MBs (Bracco) were used as standard. Site selective and stable binding of rGFP-HisTag onto the surface of metallochelating MBs was observed by confocal microscopy.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Langmuir
ISSN
0743-7463
e-ISSN
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Volume of the periodical
27
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
4829-4837
UT code for WoS article
000289321000071
EID of the result in the Scopus database
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