Immobilization of histidine-tagged proteins on monodisperse metallochelation liposomes: Preparation and study of their structure

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F11%3A00349452" target="_blank" >RIV/68378271:_____/11:00349452 - isvavai.cz</a>

Alternative codes found

RIV/61388963:_____/11:00349452 RIV/00027162:_____/11:#0000765 RIV/61989592:15110/11:10213114

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Immobilization of histidine-tagged proteins on monodisperse metallochelation liposomes: Preparation and study of their structure

Original language description

Liposomes represent a biocompatible platform for the construction of self-assembling proteoliposomes using nickel- or zinc-metallochelation. Here, we describe the design and construction of a flow-through ultrafiltration cell suitable for the preparationof monodisperse liposomes enabled for metallochelation and hence the formation of proteoliposomes. These model proteoliposomes are characterised by gel permeation chromatography and by dynamic light scattering. Transmission electron microscopy and immunogold staining are used to characterize surface bound proteins, revealing the tendency of rgp120 to form microdomains on liposome surfaces. These microdomains possess a 2D crystal-like structure that is seen more precisely by atomic force (AF) microscopy.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical Biochemistry

ISSN

0003-2697

e-ISSN

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Volume of the periodical

408

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

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UT code for WoS article

000283886600013

EID of the result in the Scopus database

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