Culture- and Quantitative IS900 Real-Time PCR-Based Analysis of the Persistence of Mycobacterium avium subspo paratuberculosis in a Controlled Dairy Cow Farm Environment
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F12%3A%230000910" target="_blank" >RIV/00027162:_____/12:#0000910 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1128/AEM.01264-12" target="_blank" >http://dx.doi.org/10.1128/AEM.01264-12</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1128/AEM.01264-12" target="_blank" >10.1128/AEM.01264-12</a>
Alternative languages
Result language
angličtina
Original language name
Culture- and Quantitative IS900 Real-Time PCR-Based Analysis of the Persistence of Mycobacterium avium subspo paratuberculosis in a Controlled Dairy Cow Farm Environment
Original language description
The aim of this study was to monitor the persistence of Mycobacterium avium subsp. paratuberculosis in environmental samples taken from a Holstein farm with a long history of clinical paratuberculosis. A herd of 606 head was eradicated, and mechanical cleaning and disinfection with chloramine B with ammonium (4%) was carried out on the farm; in the surrounding areas (on the field and field midden) lime was applied. Environmental samples were collected before and over a period of 24 months after destocking. Only one sample out of 48 (2%) examined on the farm (originating from a waste pit and collected before destocking) was positive for M. avium subsp. paratuberculosis by cultivation on solid medium (Herrold's egg yolk medium). The results using real-time quantitative PCR (qPCR) showed that a total of 81% of environmental samples with an average mean M. avium subsp. paratuberculosis cell number of 3.09 x 10(3) were positive for M. avium subsp. paratuberculosis before destocking compared
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FN - Epidemiology, infection diseases and clinical immunology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Applied and Environmental Microbiology
ISSN
0099-2240
e-ISSN
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Volume of the periodical
78
Issue of the periodical within the volume
18
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
6608-6614
UT code for WoS article
000308336400027
EID of the result in the Scopus database
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