Phenotypic characterisation of the monocyte subpopulations in healthy adult pigs and Salmonella-infected piglets by seven-colour flow cytometry

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F13%3A%230000972" target="_blank" >RIV/00027162:_____/13:#0000972 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.rvsc.2012.09.006" target="_blank" >http://dx.doi.org/10.1016/j.rvsc.2012.09.006</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.rvsc.2012.09.006" target="_blank" >10.1016/j.rvsc.2012.09.006</a>

Result language
angličtina
Original language name
Phenotypic characterisation of the monocyte subpopulations in healthy adult pigs and Salmonella-infected piglets by seven-colour flow cytometry
Original language description
The present study describes the distinct bone marrow (BM) and peripheral blood (PB) monocyte subpopulations detected by seven-colour flow cytometry. Mononuclear phagocytes were identified as viable CD172a(+) SWC8(-) CD203a(-) mononuclear leukocytes. After that, monocyte subpopulations were differentiated by using CD14, CD163 and SLA-DR markers. Four distinct monocyte subpopulations were found in the BM and PB. Based on the discovered populations two possible maturation pathways have been proposed. The first pathway was characterised by release of CD14(hi) CD163(-) SLA-DR(-) BM monocytes into the PB where they matured into CD14(low) CD163(+) SLA-DR(+) monocytes. In the alternative pathway the monocytes finalised their phenotypical maturation in the BM and then they were released into the PB as CD14(low) CD163(+) SLA-DR(+) cells. In Salmonella-infected piglets, the population of CD14(low) CD163(+) SLA-DR(+) monocytes was elevated in the BM and mesenteric lymph nodes (MLN), suggesting the
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EC - Immunology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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