Detection of allergenic parvalbumin of Atlantic and Pacific herrings in fish products by PCR

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F13%3A%230001049" target="_blank" >RIV/00027162:_____/13:#0001049 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16270/13:43873338
Result on the web
<a href="http://dx.doi.org/10.1080/19440049.2013.817024" target="_blank" >http://dx.doi.org/10.1080/19440049.2013.817024</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/19440049.2013.817024" target="_blank" >10.1080/19440049.2013.817024</a>

Result language
angličtina
Original language name
Detection of allergenic parvalbumin of Atlantic and Pacific herrings in fish products by PCR
Original language description
The conventional polymerase chain reaction (PCR) method to detect the major allergenic protein parvalbumin beta 2 of Atlantic herring (Clupea harengus) and Pacific herring (Clupea pallasii) was developed. The specific set of primers for the amplificationof the partial genomic sequence of the pvalb 2 gene encoding the main fish allergen of both herrings was designed and applied to the investigation of 24 commercial fish products. The targeted amplicon size was 189bp of pvalb 2 gene of Atlantic herring and Pacific herring. As the internal amplification control, the DNA of 18S rRNA gene for eukaryotes (141bp) was successfully used. The specificity of designed primer pair using 26 various fish species was assessed. The intrinsic detection limit was 10pg mu l(-1) of the present specific DNA. Atlantic herring or Pacific herring allergenic parvalbumins were detected in 22 investigated fish products in conformity with the package declaration. Two fish products were negative in spite of the de
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GM - Food industry
OECD FORD branch
—

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
ISSN
1944-0049
e-ISSN
—
Volume of the periodical
30
Issue of the periodical within the volume
10
Country of publishing house
GB - UNITED KINGDOM
Number of pages
5
Pages from-to
1679-1683
UT code for WoS article
000324903700001
EID of the result in the Scopus database
—

Similar results(10)