Modified hypoosmotic swelling test for the assessment of boar and bull sperm sensitivity to cryopreservation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F14%3A%230001138" target="_blank" >RIV/00027162:_____/14:#0001138 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.2754/avb201483040313" target="_blank" >http://dx.doi.org/10.2754/avb201483040313</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb201483040313" target="_blank" >10.2754/avb201483040313</a>

Result language

angličtina

Original language name

Modified hypoosmotic swelling test for the assessment of boar and bull sperm sensitivity to cryopreservation

Original language description

Routine methods for the evaluation of sperm quality are not sufficiently useful to determine the sensitivity of sperm cells to cold shock. The aim of our preliminary study was to determine whether the sperm plasma membrane integrity evaluated by modifiedhypoosmotic swelling test based on simple hypoosmotic swelling test (HOS test) and eosin-nigrosin staining could be helpful in predicting the degree of boar sperm survivability during semen cryopreservation. Ejaculates collected from 24 boars and 20 bulls were used in the experiment. Fresh ejaculates were evaluated by routine sperm analysis and a modified HOS test, and subsequently frozen. Sperm cryosurvivability was defined as the percentage of motile spermatozoa that survived the freezing process. Ahigher percentage of sperm was recovered after the thawing of semen with a higher percentage of HOS-positive and eosin-negative sperm (P < 0.01). Both indicators were found to be correlated (r = 0.707 and r = 0.705, respectively; P < 0.01

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GJ - Diseases and animal vermin, veterinary medicine

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Veterinaria Brno

ISSN

0001-7213

e-ISSN

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Volume of the periodical

83

Issue of the periodical within the volume

4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

7

Pages from-to

313-319

UT code for WoS article

000346604900006

EID of the result in the Scopus database

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