Simultaneous detection of peanut and hazelnut allergens in food matrices using multiplex PCR method

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F15%3A%230001353" target="_blank" >RIV/00027162:_____/15:#0001353 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.2754/avb201483S10S77" target="_blank" >http://dx.doi.org/10.2754/avb201483S10S77</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2754/avb201483S10S77" target="_blank" >10.2754/avb201483S10S77</a>

Result language
angličtina
Original language name
Simultaneous detection of peanut and hazelnut allergens in food matrices using multiplex PCR method
Original language description
Multiplex PCR analysis for the detection of two targeting segments of genes coding major food protein allergens as peanut (Arachis hypogaea) Ara h 1 gene and hazelnut (Corylus avellana) Cor a 1 gene was developed. Two sets of primers were designed and tested to their specificity on a broad range of ingredients. The identity of amplicons (Ara h 1- 180 bp, Cor a 1 - 258 bp) by sequencing and alignment of sequences with sequences deposited in Genbank was confirmed. When testing the specificity of designedprimer pairs on a spectrum of food ingredients, no cross reactions were detected. A potential inhibition of PCR reaction was eliminated using the universal plant primers of chloroplast gene 124 bp for the plant matrices confirmation. The intrinsic detection limit was 10 pg.ml-1 and the practical detection limit was 0.001% w/w (10 mg.kg-1) for both peanuts and hazelnuts. The method was applied to the investigation of 60 commercial food samples. The developed multiplex PCR method is cheap,
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GM - Food industry
OECD FORD branch
—

Project
<a href="/en/project/LO1218" target="_blank" >LO1218: Healthy animal as a source of wholesome food</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)