Tick salivary sialostatin L2 suppresses IFN responses in mouse dendritic cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F15%3AN0000007" target="_blank" >RIV/00027162:_____/15:N0000007 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/epdf/10.1111/pim.12162" target="_blank" >https://onlinelibrary.wiley.com/doi/epdf/10.1111/pim.12162</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/pim.12162" target="_blank" >10.1111/pim.12162</a>

Result language
angličtina
Original language name
Tick salivary sialostatin L2 suppresses IFN responses in mouse dendritic cells
Original language description
Type I interferon (IFN), mainly produced by dendritic cells(DCs), is critical in the host defence against tick-transmittedpathogens. Here, we report that salivary cysteine proteaseinhibitor from the hard tick Ixodes scapularis, sialostatin L2,affects IFN-bmediated immune reactions in mouse dendriticcells. Following IFN receptor ligation, the Janus activatedkinases/signal transducer and activator of transcription(JAK/STAT) pathway is activated. We show that sialostatinL2 attenuates phosphorylation of STATs in spleen dendriticcells upon addition of recombinant IFN-b. LPS-stimulateddendritic cells release IFN-bwhich in turn leads to the induc-tion of IFN-stimulated genes (ISG) through JAK/STATpathway activation. The induction of two ISG, interferon reg-ulatory factor 7 (IRF-7) and IP-10, was suppressed by sia-lostatin L2 in LPS-stimulated dendritic cells. Finally, theinterference of sialostatin L2 with IFN action led to theenhanced replication of tick-borne encephalitis virus in DC.In summary, we present here that tick salivary cystatin nega-tively affects IFN-bresponses which may consequentlyincrease the pathogen load after transmission via tick saliva.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30310 - Parasitology

Project
<a href="/en/project/LO1218" target="_blank" >LO1218: Healthy animal as a source of wholesome food</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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