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Mechanism of liposome formation by microfluidic mixing: the concept based on lipid bilayer fragments vesiculation

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000082" target="_blank" >RIV/00027162:_____/18:N0000082 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Mechanism of liposome formation by microfluidic mixing: the concept based on lipid bilayer fragments vesiculation

  • Original language description

    Konference DDS, Telč 2018. Liposomes are known for their biocompatible and encapsulation properties and are the most successful drug-carrier system approved by FDA. Recently new preparation technique based on nanofluidic mixing has been demonstrated a using microfluidic platform with characteristic mixing profile. The instrument NanoAssemblrTM (Precission Nanosystems, Canada) was used to prepare nanoliposomes containing metallochelating lipid 18:0 PE DTPA (Gd) to improve contrast in cryoTEM and resolve individual parts of membrane layers by cryoTEM. The size distribution was analyzed by DLS (Zetasizer Nano ZSP, Malvern Instruments, UK) and NTA (NanoSight NS500, Malvern Instruments, UK), liposomes were (114±0.3) nm in diameter with low polydispersity 0.04±0.02. By NMR (Bruker-BioSpec 94/30 USR, Bruker, GE) measurement, the use of our Gd- liposomes as theranostic has been proven. Relaxivity was much higher, around 40 mM−1·s−1 compared to common contrast agent for MRI. Using ICP-OES (ULTIMA 2, Horiba Jobin Yvon, FR) analytical measurement, the precise concentration of gadolinium was terminated to (9.30±1.49) mg·l−1. This concentration is suitable for potential in vivo experiments. Liposomes can be used as targeted diagnostic agents and will contain encapsulated APIs for therapeutic use. An important observation was the detection of lipid membrane fragments in liposomes. This work also experimentally describes the concept of liposome formulation from a lipid bilayer fragments. This new concept was proven by modern analytical methods including cryo-transmission electron microscopy technique, which allows us to observe single liposomes containing fragment and also double liposomes prior to conversion.

  • Czech name

  • Czech description

Classification

  • Type

    O - Miscellaneous

  • CEP classification

  • OECD FORD branch

    21001 - Nano-materials (production and properties)

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2018

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů