Mechanism of liposome formation by microfluidic mixing: the concept based on lipid bilayer fragments vesiculation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F18%3A73591347" target="_blank" >RIV/61989592:15110/18:73591347 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Mechanism of liposome formation by microfluidic mixing: the concept based on lipid bilayer fragments vesiculation

Original language description

Liposomes are known for their biocompatible and encapsulation properties and are the most successful drug-carrier system approved by FDA. Recently new preparation technique based on nanofluidic mixing has been demonstrated a using microfluidic platform with characteristic mixing profile. The instrument NanoAssemblrTM (Precission Nanosystems, Canada) was used to prepare nanoliposomes containing metallochelating lipid 18:0 PE DTPA (Gd) to improve contrast in cryoTEM and resolve individual parts of membrane layers by cryoTEM. The size distribution was analyzed by DLS (Zetasizer Nano ZSP, Malvern Instruments, UK) and NTA (NanoSight NS500, Malvern Instruments, UK), liposomeswere (114±0.3) nm in diameter with low polydispersity 0.04±0.02. By NMR (Bruker-BioSpec 94/30 USR, Bruker, GE) measurement, the use of our Gd-liposomes as theranostic has been proven. Relaxivity was much higher, around 40 mM−1·s−1 compared to common contrast agent for MRI. Using ICP-OES (ULTIMA 2, Horiba Jobin Yvon, FR) analytical measurement, the precise concentration of gadolinium was terminated to (9.30±1.49) mg·l−1. This concentration is suitable for potential in vivoexperiments. Liposomes can be usedas targeted diagnostic agents and will contain encapsulated APIs for therapeutic use. An important observation was the detection of lipid membrane fragments in liposomes. This work also experimentally describes the concept of liposome formulation from a lipid bilayer fragments. This new concept of was proven by modern analytical methods including cryotransmission electron microscopy technique,which allows us to observe single liposomes containing fragment and also double liposomes prior to conversion.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

10610 - Biophysics

Project

<a href="/en/project/NV15-32198A" target="_blank" >NV15-32198A: Construction of recombinant mimotopes for induction of neutralizing antibodies against HIV-1 gp120 glycoprotein using high-affinity binders approach</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

11th International Conference Drug Delivery Systems Nanotechnology for Healthcare: Progress in Recombinant Vaccines, Molecular Adjuvants, Modern Drug Delivery Systems and Cell Therapy

ISBN

978-80-907074-6-7

ISSN

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e-ISSN

neuvedeno

Number of pages

9

Pages from-to

89-97

Publisher name

nakladatelství Machovský

Place of publication

Olomouc

Event location

Telč

Event date

Jun 5, 2018

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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