Preparation of nanoliposomes by microfuidic mixing in herring-bone channel and the role of membrane fluidity in liposomes formation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F20%3A73602953" target="_blank" >RIV/61989592:15110/20:73602953 - isvavai.cz</a>
Result on the web
<a href="https://www.nature.com/articles/s41598-020-62500-2" target="_blank" >https://www.nature.com/articles/s41598-020-62500-2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-020-62500-2" target="_blank" >10.1038/s41598-020-62500-2</a>

Result language
angličtina
Original language name
Preparation of nanoliposomes by microfuidic mixing in herring-bone channel and the role of membrane fluidity in liposomes formation
Original language description
Introduction of microfuidic mixing technique opens a new door for preparation of the liposomes and lipid-based nanoparticles by on-chip technologies that are applicable in a laboratory and industrial scale. This study demonstrates the role of phospholipid bilayer fragment as the key intermediate in the mechanism of liposome formation by microfuidic mixing in the channel with “herring-bone” geometry used with the instrument NanoAssemblr. The fuidity of the lipid bilayer expressed as fuorescence anisotropy of the probe N,N,N-Trimethyl-4-(6-phenyl-1,3,5-hexatrien-1-yl) was found to be the basic parameter afecting the fnal size of formed liposomes prepared by microfuidic mixing of an ethanol solution of lipids and water phase. Both saturated and unsaturated lipids together with various content of cholesterol were used for liposome preparation and it was demonstrated, that an increase in fuidity results in a decrease of liposome size as analyzed by DLS. Gadolinium chelating lipids were used to visualize the fne structure of liposomes and bilayer fragments by CryoTEM. Experimental data and theoretical calculations are in good accordance with the theory of lipid disc micelle vesiculation.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30404 - Biomaterials (as related to medical implants, devices, sensors)

Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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