Antibacterial and antifungal activity of defensins from the Australian paralysis tick, Ixodes holocyclus
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000221" target="_blank" >RIV/00027162:_____/19:N0000221 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/19:43899505
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S1877959X19302183?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1877959X19302183?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ttbdis.2019.101269" target="_blank" >10.1016/j.ttbdis.2019.101269</a>
Alternative languages
Result language
angličtina
Original language name
Antibacterial and antifungal activity of defensins from the Australian paralysis tick, Ixodes holocyclus
Original language description
Tick innate immunity involves humoral and cellular responses. Among the humoral effector molecules in ticks are the defensins which are a family of small peptides with a conserved γ-core motif that is crucial for their antimicrobial activity. Defensin families have been identified in several hard and soft tick species. However, little is known about the presence and antimicrobial activity of defensins from the Australian paralysis tick Ixodes holocyclus. In this study the I. holocyclus transcriptome was searched for the presence of defensins. Unique and non-redundant defensin sequences were identified and designated as holosins 1 – 5. The antimicrobial activity of holosins 2 and 3 and of the predicted γ-cores of holosins 1–4 (HoloTickCores 1–4), was assessed using Gramnegative and Gram-positive bacteria as well as the fungus Fusarium graminearum and the yeast Candida albicans. All holosins had molecular features that are conserved in other tick defensins. Furthermore holosins 2 and 3 were very active against the Gram-positive bacteria Staphylococcus aureus and Listeria grayi. Holosins 2 and 3 were also active against F. graminearum and C. albicans and 5 μM of peptide abrogate the growth of these microorganisms. The activity of the synthetic γ-cores was lower than that of the mature defensins apart from HoloTickCore 2 which had activity comparable to mature holosin 2 against the Gram-negative bacterium Escherichia coli. This study reveals the presence of a multigene defensin family in I. holocyclus with wide antimicrobial activity.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30310 - Parasitology
Result continuities
Project
<a href="/en/project/EF15_003%2F0000495" target="_blank" >EF15_003/0000495: FIT (Pharmacology, Immunotherapy, nanoToxicology)</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Ticks and Tick-borne Diseases
ISSN
1877-959X
e-ISSN
1877-9603
Volume of the periodical
10
Issue of the periodical within the volume
6
Country of publishing house
DE - GERMANY
Number of pages
9
Pages from-to
101269
UT code for WoS article
000484783700018
EID of the result in the Scopus database
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