Antibacterial and antifungal activity of defensins from the Australian paralysis tick, Ixodes holocyclus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F19%3A00518834" target="_blank" >RIV/60077344:_____/19:00518834 - isvavai.cz</a>

Result on the web

<a href="https://www.sciencedirect.com/science/article/abs/pii/S1877959X19302183?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S1877959X19302183?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ttbdis.2019.101269" target="_blank" >10.1016/j.ttbdis.2019.101269</a>

Result language

angličtina

Original language name

Antibacterial and antifungal activity of defensins from the Australian paralysis tick, Ixodes holocyclus

Original language description

Tick innate immunity involves humoral and cellular responses. Among the humoral effector molecules in ticks are the defensins which are a family of small peptides with a conserved gamma-core motif that is crucial for their antimicrobial activity. Defensin families have been identified in several hard and soft tick species. However, little is known about the presence and antimicrobial activity of defensins from the Australian paralysis tick Ixodes holocyclus. In this study the I. holocyclus transcriptome was searched for the presence of defensins. Unique and non-redundant defensin sequences were identified and designated as holosins 1 5. The antimicrobial activity of holosins 2 and 3 and of the predicted y-cores of holosins 1-4 (HoloTickCores 1-4), was assessed using Gram-negative and Gram-positive bacteria as well as the fungus Fusarium graminearum and the yeast Candida albicans. All holosins had molecular features that are conserved in other tick defensins. Furthermore holosins 2 and 3 were very active against the Gram-positive bacteria Staphylococcus aureus and Listeria grayi. Holosins 2 and 3 were also active against F. graminearum and C. albicans and 5 mu M of peptide abrogate the growth of these microorganisms. The activity of the synthetic gamma-cores was lower than that of the mature defensins apart from HoloTickCore 2 which had activity comparable to mature holosin 2 against the Gram-negative bacterium Escherichia coli. This study reveals the presence of a multigene defensin family in I. holocyclus with wide antimicrobial activity.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30303 - Infectious Diseases

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Ticks and Tick-Borne Diseases

ISSN

1877-9603

e-ISSN

—

Volume of the periodical

10

Issue of the periodical within the volume

6

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

101269

UT code for WoS article

000484783700018

EID of the result in the Scopus database

2-s2.0-85070885430