Specific alterations of sphingolipid metabolism identified in EpCAM-positive cells isolated from human colon tumors
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F20%3AN0000084" target="_blank" >RIV/00027162:_____/20:N0000084 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/20:00537790 RIV/00216224:14110/20:00116195 RIV/61989592:15110/20:73602800 RIV/00098892:_____/20:N0000187
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S1388198120301347?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1388198120301347?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bbalip.2020.158742" target="_blank" >10.1016/j.bbalip.2020.158742</a>
Alternative languages
Result language
angličtina
Original language name
Specific alterations of sphingolipid metabolism identified in EpCAM-positive cells isolated from human colon tumors
Original language description
"Metabolic reprogramming leading to alterations in lipid metabolism and lipid-mediated signaling may contribute to colorectal cancer (CRC) development and progression. We hypothesized that a detailed description of changes in sphingolipidome of specific cellular subpopulation residing in colon cancer tissue may help to discriminate between normal and transformed colon epithelial cells. Using HPLC-mass spectrometry, we analyzed the EpCAM-positive cells isolated from tumor and adjacent non-tumor tissues of colon cancer patients, aiming to identify potential lipid biomarkers specific for CRC. We then employed RT-qPCR-based methodology in order to analyze expression of SL metabolism-related genes in the isolated EpCAM-positive tumor cells and/or in unseparated colon tumor tissues. We observed significant changes in sphingolipid (SL) species in the EpCAM-positive tumor cells, with the accumulation of lactosylceramide (LacCer) being the most prominent. B4GALT5 and B4GALT6 genes were identified as two potential gene candidates contributing to LacCer accumulation. We further identified additional genes of SL and fatty acid metabolism (e.g. SPHK1, GBA2, NEU3, GLA, FASN, PLA2G10), which were significantly altered in colon tumor tissue. The present results indicate that the EpCAM-positive cells are a major contributor to LacCer accumulation in CRC tissue, and may help to identify novel CRC specific lipid/gene biomarkers. "
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30204 - Oncology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
ISSN
1388-1981
e-ISSN
1879-2618
Volume of the periodical
1865
Issue of the periodical within the volume
9
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
11
Pages from-to
"158742"
UT code for WoS article
000552710900012
EID of the result in the Scopus database
2-s2.0-85085313631