Identification of biofilm hotspots in a meat processing environment: detection of spoilage bacteria in multi-species biofilms

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F20%3AN0000119" target="_blank" >RIV/00027162:_____/20:N0000119 - isvavai.cz</a>

Result on the web

<a href="https://reader.elsevier.com/reader/sd/pii/S0168160520301628?token=F4558C705A2C22CE93F45C493AB81820B05FDD9422B5F4A65DB78BC458AC54A058D6AA92B30B896A4D3A8F6C6C5875C3" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S0168160520301628?token=F4558C705A2C22CE93F45C493AB81820B05FDD9422B5F4A65DB78BC458AC54A058D6AA92B30B896A4D3A8F6C6C5875C3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijfoodmicro.2020.108668" target="_blank" >10.1016/j.ijfoodmicro.2020.108668</a>

Result language

angličtina

Original language name

Identification of biofilm hotspots in a meat processing environment: detection of spoilage bacteria in multi-species biofilms

Original language description

Biofilms are comprised of microorganisms embedded in a selfproduced matrix that normally adhere to a surface. In the food processing environment, they are suggested to be a source of contamination leading to food spoilage or the transmission of food-borne pathogens. To date, research has mainly focused on the presence of (biofilm-forming) bacteria within food processing environments, without measuring the associated biofilm matrix components. Here, we assess the presence of biofilms within a meat processing environment by the detection of microorganisms and at least two biofilm matrix components. Sampling included 47 food contact surfaces and 61 nonfood contact surfaces from eleven rooms within an Austrian meat processing plant, either during operation or after cleaning and disinfection. The 108 samples were analysed for the presence of microorganisms by cultivation and targeted quantitative real-time PCR based on16S rRNA. Furthermore, the presence of the major matrix components carbohydrates, extracellular DNA and proteins was evaluated. Overall, we identified ten biofilm hotspots, among them seven of which were sampled during operation and three after cleaning and disinfection. Five biofilms were detected on food contact surfaces (cutters and associated equipment and a screw conveyor) and five on non-food contact surfaces (drains and water hoses) resulting in 9.3 % of the sites being classified as biofilm-positive. From these biofilm positive samples we cultivated bacteria of 29 different genera. The most prevalent bacteria belonged to the genera Brochothrix, (present in 80 % of biofilms), Pseudomonas and Psychrobacter, (isolated from 70 % biofilms). From each biofilm we isolated bacteria from at four to 12 different genera, indicating the presence of multi-species biofilms. This work ultimately determined the presence of multi-species biofilms within the meat processing environment, thereby identifying various sources of potential contamination. The knowledge gained about the presence and composition of biofilms (i.e.chemical and microbiological)will help to prevent and reduce biofilm formation within food processing environments.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/QK1910121" target="_blank" >QK1910121: Persistence of selected foodborne-diseases agents, hygienic indicators and the possibilities of their elimination from the environment of food processing plants</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY

ISSN

0168-1605

e-ISSN

1879-3460

Volume of the periodical

328

Issue of the periodical within the volume

SEP 2 2020

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

"108668"

UT code for WoS article

000539331300006

EID of the result in the Scopus database

2-s2.0-85085374250