Microcomputed tomographic, biomechanical and histological analyses of lumbar interbody fusion with iliac crest bone graft in pig model
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000054" target="_blank" >RIV/00027162:_____/21:N0000054 - isvavai.cz</a>
Result on the web
<a href="https://actavet.vfu.cz/90/1/0069/" target="_blank" >https://actavet.vfu.cz/90/1/0069/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2754/avb202190010069" target="_blank" >10.2754/avb202190010069</a>
Alternative languages
Result language
angličtina
Original language name
Microcomputed tomographic, biomechanical and histological analyses of lumbar interbody fusion with iliac crest bone graft in pig model
Original language description
The main goal of this study was to assess the progress of vertebral stability after lumbar interbody fusion related to microcomputed tomography (micro CT), biomechanical analysis, and histological assessment towards spine fusion. Twelve male pigs were used; each underwent L2-3 discectomy and implantation of an iliac crest bone graft in two groups; six spines were harvested eight weeks (A1) and six spines 16 weeks (A2) after surgery (7 native spines for biomechanical analysis). The CT was performed by GE phoenix datos|x 2.0 with a sample drift correction. The samples were divided according to fusion quality. Biomechanical evaluation was carried out on the MTS Mini Bionix testing system. In the nondestructive mode, three cycles of pure bending moments were applied (5 Nm load limit) at a rate of 20 °/min in flexion (+40 °) and extension (-40 °). Two representative histological sections from four samples were obtained (A1, n = 2; A2, n = 2); areas of mature bone were quantified. In micro CT, better results were achieved in group A2 (not significant). Eight weeks after the operation, flexural stiffness decreased to 48% of its initial value for native cadavers (P < 0.05); after 16 weeks it was comparable to native cadavers, demonstrating the suitability of the implanted graft (P < 0.05). The newly formed bone tissue occupied an average area of 94.205 mm2 (A1) and 26.240 mm2 (A2). It was confirmed that microCT, biomechanical analysis, and histological assessment are technically feasible and suitable for the evaluation of results of other methods of large bone defect treatment.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30211 - Orthopaedics
Result continuities
Project
<a href="/en/project/NV17-31276A" target="_blank" >NV17-31276A: Comprehensive pre-clinical evaluation of lateral lumbar spine fusion with hybrid biodegradable nanocomposite porous implant.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ACTA VETERINARIA BRNO
ISSN
0001-7213
e-ISSN
1801-7576
Volume of the periodical
90
Issue of the periodical within the volume
1
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
10
Pages from-to
69-75
UT code for WoS article
000654628800009
EID of the result in the Scopus database
2-s2.0-85104823198