EXPERIMENTAL CONJUGATED VACCINE AGAINST STREPTOCOCCUS SUIS

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000155" target="_blank" >RIV/00027162:_____/21:N0000155 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/21:00124405

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

EXPERIMENTAL CONJUGATED VACCINE AGAINST STREPTOCOCCUS SUIS

Original language description

Streptococcus suis is an economically significant pathogen that causes high morbidity and mortality negatively affecting the swine industry worldwide. Effective vaccines against S. suis have not been developed yet. Currently, only autogenous vaccines are available, but their protection is limited. In this study, we aim to develop experimental vaccines that would protect pigs against S. suis. Two types of conjugated vaccines were prepared by chemical conjugation of capsular polysaccharide (CPS), isolated from S. suis, to either ovalbumin (OVA) or cross-reactive material 197 (CRM197) as a carrier proteins. Immunogenicity of prepared conjugated vaccines was tested on mouse model. Six groups of ten mice were vaccinated with OVA and CRM197 proteins alone, with the CPS-OVA and CPS-CRM197 conjugates, with CPS alone and last group of ten mice were vaccinated with DPBS as a control group. The presence of antibodies in serum of immunized mice was confirmed by the western blot method, while ELISA method was then used to determine the concentration of antibodies. ELISA proved that groups of mice immunized with the conjugates induced a high immune response to the capsular polysaccharide as antigen, whereas the group of mice immunized with capsular polysaccharide alone did not induce any immune response. Groups of mice immunized with the conjugates also induced a greater immune response to the respective carrier proteins than groups of mice immunized with these proteins only. These experimental conjugate vaccines will be used for immunization and challenge of pigs. This project was supported by the Ministry of Agriculture (QK1810193 and RO0518).

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

<a href="/en/project/QK1810193" target="_blank" >QK1810193: Modern methods of diagnosis, therapy and prevention of Streptococcus suis infections as a tool for designing of targeted control programs in pig herds</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů