Raw bovine milk as a reservoir of yeast with virulence factors and decreased susceptibility to antifungal agents
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000168" target="_blank" >RIV/00027162:_____/21:N0000168 - isvavai.cz</a>
Result on the web
<a href="https://academic.oup.com/mmy/article-abstract/59/10/1032/6309310?redirectedFrom=fulltext" target="_blank" >https://academic.oup.com/mmy/article-abstract/59/10/1032/6309310?redirectedFrom=fulltext</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/mmy/myab036" target="_blank" >10.1093/mmy/myab036</a>
Alternative languages
Result language
angličtina
Original language name
Raw bovine milk as a reservoir of yeast with virulence factors and decreased susceptibility to antifungal agents
Original language description
In recent years, increased rates of yeast infections in humans and animals are recognized worldwide. Considering that animals can represent a source of yeast infections for humans, knowing the antifungal susceptibility profile of yeast isolates from milk and evaluating their pathogenic potential would be of great medical importance. Therefore, the aim of this survey was to study yeast diversity in milk samples, determine hemolytic and phospholipase activities of isolates and establish minimal inhibition concentration (MIC) for fluconazole, voriconazole and flucytosine, using microdilution method. Out of 66 yeast isolates from raw milk samples of 910 subclinically infected cows, 26 different yeast species were determined based on sequencing of the D1/D2 region and ITS region. Among them, Pichia kudriavzevii (9; Candida krusei), Kluyveromyces marxianus (8; Candida kefyr) and Debaryomyces hansenii (8; Candida famata) were the most commonly identified. Hemolysin and phospholipase activity was observed in more than half percent of isolates, separately. The elevated MIC for fluconazole above 4 mg/ml was determined in 16 isolates of 11 species except for intrinsically resistant isolates of P. kudriavzevii. The findings of this study demonstrate that yeast isolates from raw milk have the potential to express virulence attributes such as hemolysin and phospholipase and additionally, some of these strains showed elevated MIC to fluconazole or to flucytosin.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40200 - Animal and Dairy science
Result continuities
Project
<a href="/en/project/QK1910092" target="_blank" >QK1910092: Non-bacterial agents of mastitis and their influence on the quality and technological properties of milk</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Medical Mycology
ISSN
1369-3786
e-ISSN
1460-2709
Volume of the periodical
59
Issue of the periodical within the volume
10
Country of publishing house
GB - UNITED KINGDOM
Number of pages
9
Pages from-to
1032–1040
UT code for WoS article
000709571800011
EID of the result in the Scopus database
2-s2.0-85117388473