Overexpression of CD44v8-10 in Colon Polyps—A Possible Key to Early Diagnosis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000209" target="_blank" >RIV/00027162:_____/21:N0000209 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11150/21:10435167 RIV/65269705:_____/21:00074367 RIV/61989592:15110/21:73607577 RIV/00216224:14110/21:00122438
Result on the web
<a href="https://www.por-journal.com/articles/10.3389/pore.2021.614281/full" target="_blank" >https://www.por-journal.com/articles/10.3389/pore.2021.614281/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/pore.2021.614281" target="_blank" >10.3389/pore.2021.614281</a>
Alternative languages
Result language
angličtina
Original language name
Overexpression of CD44v8-10 in Colon Polyps—A Possible Key to Early Diagnosis
Original language description
Background and aims: The majority of colorectal cancers arise from detectable adenomatous or serrated lesions. Here we demonstrate how deregulated alternative splicing of CD44 gene in diseased colon mucosa results in downregulation of standard isoform of CD44 gene (CD44s) and upregulation of variant isoform CD44v8-10. Our aim is to show that upregulation of CD44v8-10 isoform is a possible marker of precancerous lesion in human colon. Methods: We analysed pairs of fresh biopsy specimen of large intestine in a cohort of 50 patients. We studied and compared alternative splicing profile of CD44 gene in colon polyps and adjoined healthy colon mucosa. We performed end-point and qRT PCR, western blotting, IHC staining and flow cytometry analyses. Results: We detected more than five-fold overexpression of CD44v8-10 isoform and almost twenty-fold downregulation of standard isoform CD44s in colon polyps compared to adjoined healthy tissue with p = 0.018 and p < 0.001 in a cohort of 50 patients. Our results also show that aberrant splicing of CD44 occurs in both biologically distinct subtypes of colorectal adenoma possibly in ESRP-1 specific manner. Conclusion: 92% of the colon polyp positive patients overexpressed CD44v8-10 isoform in their colon polyps while only 36% of them had positive fecal occult blood test which is currently a standard non-invasive screening technique. Impact: We believe that our results are important for further steps leading to application of CD44v8-10 isoform as a biomarker of colorectal precancerosis in non-invasive detection. Early detection of colon precancerosis means successful prevention of colorectal carcinoma.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PATHOLOGY & ONCOLOGY RESEARCH
ISSN
1219-4956
e-ISSN
1532-2807
Volume of the periodical
27
Issue of the periodical within the volume
MAR 30 2021
Country of publishing house
CH - SWITZERLAND
Number of pages
11
Pages from-to
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UT code for WoS article
000639342800001
EID of the result in the Scopus database
2-s2.0-85104123998