Antimicrobial susceptibility testing of thermally stabilized endolysins
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000240" target="_blank" >RIV/00027162:_____/21:N0000240 - isvavai.cz</a>
Result on the web
<a href="http://symma.cz/hojeniran/down/hojeniran2021_program.pdf" target="_blank" >http://symma.cz/hojeniran/down/hojeniran2021_program.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Antimicrobial susceptibility testing of thermally stabilized endolysins
Original language description
Endolysins are phage-encoded peptidoglycan hydrolases that play an essential role in the release of the phage progeny to the environment while destroying the bacterial cell. Endolysins display strong lytic activity, particularly in Gram-positive bacteria with no protective outer cell membrane. In our study, we aim to improve the thermal stability of these enzymes to achieve better antimicrobial properties. Existing endolysin F1 was altered employing protein engineering, and stabilizing mutations were introduced into the structure. An increase in the thermal stability of novel enzymes was verified by a thermal shift assay. Antimicrobial susceptibility testing was performed by recording bacterial growth curves over 24-hour cultivation of two methicillin-resistant Staphylococcus aureus strains for each enzyme variant in concentrations ranging from 0.78 to 50 µg/mL. These measurements confirmed that the introduction of stabilizing mutations improved antimicrobial properties. Modified endolysins proved to be more stable in time (the antimicrobial effect lasted longer, up to 20 hours), and lower concentrations were needed to reduce bacterial counts (3 µg/mL). These results will help the effort to use endolysins in the fields of medicine and biotechnology.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/NV19-05-00214" target="_blank" >NV19-05-00214: Study of therapeutic application of antibacterial wound dressing for skin and soft tissue infections in epidemiologically relevant strains of S. aureus - methicillin resistant.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů