Comparison of the efficiency, safety and immune response to oil-based adjuvant after dermal vs intramuscular immunization: the Actinobacillus pleuropneumoniae model

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F22%3AN0000160" target="_blank" >RIV/00027162:_____/22:N0000160 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Comparison of the efficiency, safety and immune response to oil-based adjuvant after dermal vs intramuscular immunization: the Actinobacillus pleuropneumoniae model

Original language description

Actinobacillus pleuropneumoniae (App) is an aetiological agent of swine pleuropneumonia, disease of the respiratory tract of pigs responsible for substantial losses in the pig industry worldwide. Vaccination programmes contribute to improving animal health. Route of vaccine delivery can greatly impact the immunogenicity, efficacy and safety of the vaccine. Vaccine efficacy can also be increased and optimised by the use of an appropriate adjuvant. In this study, piglets were immunised transdermally (TD), intradermally (ID) and intramuscularly (IM) with the same doses of antigen in combination with emulsion adjuvant Montanide™ ISA 201 VG (Seppic, France) and subsequently exposed to the experimental infection induced by APP. The intensity of antibody response including the characterisation of antibody isotypes and cellular response were compared with the intensity of adverse local reactions and level of protection against challenge infection induced by App. The monitoring of the local reaction at the injection site after each administration, showed that IM route was less reactogenic than the two others. Moreover, in terms of efficacy, IM injection induced higher App9-specific isotypes IgG and IgM, while ID route induced a slightly higher titre of IgG2 isotype-specific antibodies against ApxI. Analysis of App9-specific isotypes IgG1 and IgG2 revealed a close immunological profile between IM and ID routes. Level of ApxI-specific IgM isotype was similar in all groups. The concentration of IFN-γ from peripheral blood after in vitro restimulation with the specific antigen, was only increased in the IM group before challenge (D35) and two weeks after (D49). However, the gross pulmonary lesions observed after challenge at D49 was much less important in the ID group compared to other routes of administration. Taken together, ID vaccine was more reactogenic and slightly less immunogenic than the IM vaccine, its protection effectiveness seemed to be superior. These results suggest that ID administration of vaccines is an interesting approach.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů