First Evidence of the Presence of the Causative Agent of Caseous Lymphadenitis—Corynebacterium pseudotuberculosis in Dairy Products Produced from the Milk of Small Ruminants

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F22%3AN0000169" target="_blank" >RIV/00027162:_____/22:N0000169 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/2076-0817/11/12/1425" target="_blank" >https://www.mdpi.com/2076-0817/11/12/1425</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/pathogens11121425" target="_blank" >10.3390/pathogens11121425</a>

Result language

angličtina

Original language name

First Evidence of the Presence of the Causative Agent of Caseous Lymphadenitis—Corynebacterium pseudotuberculosis in Dairy Products Produced from the Milk of Small Ruminants

Original language description

This study focused on the detection and quantification of zoonotic bacteria and on the presence of enterotoxin genes in milk and dairy products from sheep and goat farms in the Czech Republic using quantitative real-time PCR (qPCR) and multiplex PCR (PCR). The presence of Corynebacterium pseudotuberculosis (CP), Mycobacterium avium subsp. paratuberculosis (MAP), Listeria monocytogenes, Staphylococcus aureus, S. aureus enterotoxin genes and methicillin-resistant Staphylococcus aureus (MRSA) was determined in 18 milk samples, 28 fresh cheeses, 20 ripened cheeses and 14 yoghurts. The serological status of the herds in relation to CP and MAP was taken into account. The most frequently detected bacterium was S. aureus (48.8 %), and subsequent PCR revealed 11 MRSA positive samples. The S. aureus enterotoxin genes seg, sei and sec were detected in two goat cheeses. CP (8.8 %) and MAP (13.8 %) were detected by qPCR on two different seropositive farms. Cultivation of qPCR positive CP samples on agar plates supplemented with potassium tellurite showed the presence of viable bacterium. This is the first evidence of CP in dairy products. The results obtained confirmed the necessity of monitoring the infectious status of dairy animals and rapid diagnosis of bacterial pathogens in milk and dairy products.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

<a href="/en/project/QK1910082" target="_blank" >QK1910082: Addressing the problem of the occurrence of bacterial, protozoan and viral zoonotic agents in small ruminant breeds</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Pathogens

ISSN

2076-0817

e-ISSN

2076-0817

Volume of the periodical

11

Issue of the periodical within the volume

12

Country of publishing house

CH - SWITZERLAND

Number of pages

12

Pages from-to

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UT code for WoS article

000902988100001

EID of the result in the Scopus database

2-s2.0-85144644060