Antimicrobial effect of endolysins LYSDERM-S and LYSDERM-T1 and endolysin-ubiquitin combination on methicillin-resistant Staphylococcus aureus
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F23%3AN0000008" target="_blank" >RIV/00027162:_____/23:N0000008 - isvavai.cz</a>
Alternative codes found
RIV/00159816:_____/23:00078674 RIV/00216224:14110/23:00134618
Result on the web
<a href="https://link.springer.com/article/10.1007/s11756-022-01282-6" target="_blank" >https://link.springer.com/article/10.1007/s11756-022-01282-6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11756-022-01282-6" target="_blank" >10.1007/s11756-022-01282-6</a>
Alternative languages
Result language
angličtina
Original language name
Antimicrobial effect of endolysins LYSDERM-S and LYSDERM-T1 and endolysin-ubiquitin combination on methicillin-resistant Staphylococcus aureus
Original language description
Bacterial resistance is a major issue in the modern world, and Staphylococcus aureus is one of these well-known multi-resistant species. Staphylococcal infections are one of the leading causes of infection in humans and are becoming more challenging to treat by conventional methods. Endolysins, a novel class of antibacterial agents, are bacteriophage-encoded lytic enzymes capable of degrading peptidoglycan and thus able to kill bacteria. This study aimed to study endolysin LysF1 and newly prepared thermally stabilized endolysin LysT1 both with fused ubiquitin and determine its role in protein expression and antibacterial activity. The results showed that fused endolysin-ubiquitin proteins did not exceed the antimicrobial effect of endolysins alone, but cleaved endolysin-ubiquitin proteins possessed longer lasting antimicrobial effect than endolysin alone. The endolysin LysT1 with higher thermal stability showed a better antimicrobial effect. Further, we showed that ubiquitin alone possesses antimicrobial properties. Minimal inhibitory and bactericidal concentrations (MIC and MBC) were assessed and confirmed that ubiquitin is able to increase the antimicrobial potential of endolysins. Endolysin or endolysin-ubiquitin combination could serve as an alternative to well-established antimicrobial therapy for methicillin-resistant S. aureus infections.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biologia
ISSN
0006-3088
e-ISSN
1336-9563
Volume of the periodical
78
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
601-608
UT code for WoS article
000894986000002
EID of the result in the Scopus database
2-s2.0-85143397420