Early onset of apc/c activity renders sac inefficient in mouse embryos
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F24%3AN0000021" target="_blank" >RIV/00027162:_____/24:N0000021 - isvavai.cz</a>
Alternative codes found
RIV/67985904:_____/24:00584848 RIV/00216224:14310/24:00135724
Result on the web
<a href="https://www.frontiersin.org/articles/10.3389/fcell.2024.1355979/abstract" target="_blank" >https://www.frontiersin.org/articles/10.3389/fcell.2024.1355979/abstract</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fcell.2024.1355979" target="_blank" >10.3389/fcell.2024.1355979</a>
Alternative languages
Result language
angličtina
Original language name
Early onset of apc/c activity renders sac inefficient in mouse embryos
Original language description
Control mechanisms of spindle assembly and chromosome segregaDon are vital for prevenDng aneuploidy during cell division. The mammalian germ cells and embryos are prone to chromosome segregaDon errors and resulDng aneuploidy is a major cause of terminaDon of development or severe developmental disorders. Here we focused on early mouse embryos, and using combinaDon of methods involving microinjecDon, immunodetecDon and confocal live cell imaging, we concentrated on the Spindle Assembly Checkpoint (SAC) and Anaphase PromoDng Complex/Cyclosome (APC/C). These are two important mechanisms cooperaDng during mitosis to ensure accurate chromosome segregaDon, and assessed their acDvity during the first two mitoses a[er ferDlizaDon. Our results showed, that in zygotes and 2-cell embryos, the SAC core protein Mad1 shows very low levels on kinetochores in comparison to oocytes and its interacDon with chromosomes is restricted to a short Dme interval a[er nuclear membrane disassembly (NEBD). Exposure of 2-cell embryos to low levels of spindle poison does not prevent anaphase, despite the spindle damage induced by the drug. And lastly, the APC/C is acDvated coincidentally with NEBD, before the spindle assembly compleDon. This early onset of APC/C acDvity, together with precocious relocalizaDon of Mad1 from chromosomes, prevents proper surveillance of spindle assembly by SAC. The results contribute to the understanding of the origin of aneuploidy in early embryos.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40203 - Husbandry
Result continuities
Project
<a href="/en/project/GA20-25850S" target="_blank" >GA20-25850S: Control of anaphase entry during early development</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Cell and Developmental Biology
ISSN
2296-634X
e-ISSN
2296-634X
Volume of the periodical
12
Issue of the periodical within the volume
2024
Country of publishing house
CH - SWITZERLAND
Number of pages
13
Pages from-to
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UT code for WoS article
001190896200001
EID of the result in the Scopus database
2-s2.0-85188590577