Intracellular accumulation of bilirubin as a defense mechanism against increased oxidative stress

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064165%3A_____%2F12%3A13056" target="_blank" >RIV/00064165:_____/12:13056 - isvavai.cz</a>

Alternative codes found

RIV/67985823:_____/12:00390672 RIV/00216208:11110/12:13056

Result on the web

<a href="http://dx.doi.org/10.1016/j.biochi.2012.04.026" target="_blank" >http://dx.doi.org/10.1016/j.biochi.2012.04.026</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Intracellular accumulation of bilirubin as a defense mechanism against increased oxidative stress

Original language description

Antioxidant, anti-inflammatory and anti-atherogenic effects have been associated with elevations of unconjugated bilirubin (UCB) in serum and with the induction of heme oxygenase-1 (HO-1), the rate-limiting enzyme in UCB synthesis. The aim of this studywas to investigate the intracellular metabolism and antioxidant properties of UCB in human hepatoblastoma HepG2 cells and tissues of Wistar rats exposed to oxidative stressors and lipopolysaccharide (LPS), respectively. Intracellular UCB concentrations in HepG2 cells correlated with its levels in culture media (p < 0.001) and diminished lipid peroxidation in a dose-dependent manner (p < 0.001). Moreover, induction of HO-1 with sodium arsenite led to 2.4-fold (p = 0.01) accumulation of intracellular UCBover basal level while sodium azide-derived oxidative stress resulted in a 60% drop (p < 0.001). This decrease was ameliorated by UCB elevation in media or by simultaneous induction of HO-1. In addition, hyperbilirubinemia and liver HO-1

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/NT11327" target="_blank" >NT11327: Role of the Heme Oxygenase in the Pathogenesis of Cholestasis</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biochimie

ISSN

0300-9084

e-ISSN

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Volume of the periodical

94

Issue of the periodical within the volume

8

Country of publishing house

FR - FRANCE

Number of pages

7

Pages from-to

1821-1827

UT code for WoS article

000306209000022

EID of the result in the Scopus database

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