The Extent of Intracellular Accumulation of Bilirubin Determines Its Anti- or Pro-Oxidant Effect
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F20%3A10418127" target="_blank" >RIV/00216208:11110/20:10418127 - isvavai.cz</a>
Alternative codes found
RIV/00064165:_____/20:10418127
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=lG_8VoOECJ" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=lG_8VoOECJ</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms21218101" target="_blank" >10.3390/ijms21218101</a>
Alternative languages
Result language
angličtina
Original language name
The Extent of Intracellular Accumulation of Bilirubin Determines Its Anti- or Pro-Oxidant Effect
Original language description
Background: Severe hyperbilirubinemia can cause permanent neurological damage in particular in neonates, whereas mildly elevated serum bilirubin protects from various oxidative stress-mediated diseases. The present work aimed to establish the intracellular unconjugated bilirubin concentrations (iUCB) thresholds differentiating between anti- and pro-oxidant effects. Methods: Hepatic (HepG2), heart endothelial (H5V), kidney tubular (HK2) and neuronal (SH-SY5Y) cell lines were exposed to increasing concentration of bilirubin. iUCB, cytotoxicity, intracellular reactive oxygen species (ROS) concentrations, and antioxidant capacity (50% efficacy concentration (EC50)) were determined. Results: Exposure of SH-SY5Y to UCB concentration > 3.6 mu M (iUCB of 25 ng/mg) and >15 mu M in H5V and HK2 cells (iUCB of 40 ng/mg) increased intracellular ROS production (p < 0.05). EC50 of the antioxidant activity was 21 mu M (iUCB between 5.4 and 21 ng/mg) in HepG2 cells, 0.68 mu M (iUCB between 3.3 and 7.5 ng/mg) in SH-SY5Y cells, 2.4 mu M (iUCB between 3 and 6.7 ng/mg) in HK2 cells, and 4 mu M (iUCB between 4.7 and 7.5 ng/mg) in H5V cells. Conclusions: In all the cell lines studied, iUCB of around 7 ng/mg protein had antioxidant activities, while iUCB > 25 ng/mg protein resulted in a prooxidant and cytotoxic effects. UCB metabolism was found to be cell-specific resulting in different iUCB.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/NV18-07-00342" target="_blank" >NV18-07-00342: Studies of the bilirubin degradation products formed during phototherapy of neonatal jaundice</a><br>
Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Sciences [online]
ISSN
1422-0067
e-ISSN
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Volume of the periodical
21
Issue of the periodical within the volume
21
Country of publishing house
CH - SWITZERLAND
Number of pages
17
Pages from-to
8101
UT code for WoS article
000589039400001
EID of the result in the Scopus database
2-s2.0-85095578945