Immunohistochemical Analysis of Collagen IV and Laminin Expression in Spontaneous Melanoma Regression in the Melanoma-Bearing Libechov Minipig

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064173%3A_____%2F15%3A%230000457" target="_blank" >RIV/00064173:_____/15:#0000457 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/15:00443826 RIV/67985904:_____/15:00443826 RIV/00216208:11310/15:10314685 RIV/00216208:11120/15:43909445

Result on the web

<a href="http://dx.doi.org/10.1267/ahc.14020" target="_blank" >http://dx.doi.org/10.1267/ahc.14020</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1267/ahc.14020" target="_blank" >10.1267/ahc.14020</a>

Result language

angličtina

Original language name

Immunohistochemical Analysis of Collagen IV and Laminin Expression in Spontaneous Melanoma Regression in the Melanoma-Bearing Libechov Minipig

Original language description

Spontaneous regression (SR) of human melanoma is a rare, well-documented phenomenon that is not still fully understood. Its detailed study cannot be performed in patients due to ethical reasons. Using the Melanoma-bearing Libechov Minipig (MeLiM) animals of various ages (from 3 weeks to 8 months) we implemented a long-term monitoring of melanoma growth and SR. We focused on immunohistochemical detection of two important extracellular matrix proteins, collagen IV and laminin, which are associated with cancer. We showed that SR of melanoma is a highly dynamic process. The expression of collagen IV and laminin correlated with changes in population of melanoma cells. Tumours of 3-week-old animals consisted primarily of melanoma cells with a granular expression of collagen IV and laminin around them. Thereafter, melanoma cells were gradually destroyed and tumour tissue was rebuilt into the connective tissue. Collagen IV expression slightly increased in tumours of 10-week-old pigs showing extracellular fibrous appearance. In tumours of older animals, areas lacking melanoma cells demonstrated a low expression and areas still containing melanoma cells a high expression of both proteins. We considered the age of 10 weeks as a turning point in the transition between tumour growth and SR of the MeLiM melanoma.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EC - Immunology

OECD FORD branch

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Project

<a href="/en/project/ED2.1.00%2F03.0124" target="_blank" >ED2.1.00/03.0124: ExAM Experimental Animal Models</a><br>

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Histochemica et Cytochemica

ISSN

0044-5991

e-ISSN

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Volume of the periodical

48

Issue of the periodical within the volume

1

Country of publishing house

JP - JAPAN

Number of pages

12

Pages from-to

15-26

UT code for WoS article

000354928000003

EID of the result in the Scopus database

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