Etiopathogenesis of adolescent idiopathic scoliosis: Expression of melatonin receptors 1A/1B, calmodulin and estrogen receptor 2 in deep paravertebral muscles revisited

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064173%3A_____%2F16%3AN0000019" target="_blank" >RIV/00064173:_____/16:N0000019 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11130/16:10333061 RIV/00064203:_____/16:10333061 RIV/00216208:11120/16:43912352

Result on the web

<a href="http://dx.doi.org/10.3892/mmr.2016.5927" target="_blank" >http://dx.doi.org/10.3892/mmr.2016.5927</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3892/mmr.2016.5927" target="_blank" >10.3892/mmr.2016.5927</a>

Result language

angličtina

Original language name

Etiopathogenesis of adolescent idiopathic scoliosis: Expression of melatonin receptors 1A/1B, calmodulin and estrogen receptor 2 in deep paravertebral muscles revisited

Original language description

The pathogenesis of adolescent idiopathic scoliosis (AIS), including the associated local changes in deep paravertebral muscles, is poorly understood. The asymmetric expression of several molecules involved in the melatonin signaling pathway, including melatonin receptors 1A/1B (MTNR1A/MTNR1B), estrogen receptor 2 (ESR2) and calmodulin (CALM1), has previously been suggested to be associated with AIS. However, this hypothesis is based on single studies in which the data were obtained by different methodological approaches. Therefore, to evaluate the symmetry of the mRNA expression levels of these molecules, 18 patients with AIS and 10 non-scoliotic controls were enrolled in the present study. Muscle biopsy samples from deep paraspinal muscles (from the convexity and concavity of the scoliotic curve in patients with AIS, or from the left and right sides in controls) were obtained during spinal surgery. For each sample, the relative mRNA expression levels of MTNR1A, MTNR1B, CALM1 and ESR2 were analyzed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and were quantified according to the quantification cycle method. The results indicated that the mRNA expression levels of none of the investigated molecules were significantly different between samples obtained from the convex and concave side of the scoliotic curve in patients with AIS. In addition, no difference in expression was detected between the patients with AIS and the controls. With regards to MTNR1A and MTNR1B, their expression was very weak in paravertebral muscles, and in the majority of cases their expression could not be detected by repeated RT-qPCR analysis. Therefore, these data do not support the previously suggested role of the asymmetric expression of molecules involved in the melatonin signaling pathway in deep paravertebral muscles in the pathogenesis of AIS.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

—

Project

<a href="/en/project/NT13693" target="_blank" >NT13693: Morphological, histochemical and electrophysiological changes in the chest wall muscles in patients with idiopathic scoliosis and their potential role in the etiopathogenesis of the disease.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular Medicine Reports

ISSN

1791-2997

e-ISSN

—

Volume of the periodical

14

Issue of the periodical within the volume

6

Country of publishing house

GR - GREECE

Number of pages

6

Pages from-to

5719-5724

UT code for WoS article

000391076200039

EID of the result in the Scopus database

2-s2.0-84999106979