Changes in telomere length and mitochondrial DNA copy number in the colorectal adenoma-carcinoma sequence

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064190%3A_____%2F26%3A10001445" target="_blank" >RIV/00064190:_____/26:10001445 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1093/mutage/geag009" target="_blank" >https://doi.org/10.1093/mutage/geag009</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/mutage/geag009" target="_blank" >10.1093/mutage/geag009</a>

Result language

angličtina

Original language name

Changes in telomere length and mitochondrial DNA copy number in the colorectal adenoma-carcinoma sequence

Original language description

Colorectal adenomas are anomalous growths of the intestinal epithelium and are considered precursors to colorectal cancer (CRC). Identifying early-stage CRC biomarkers is essential for reducing its high mortality rate. This study hypothesizes that the association of telomere length (TL) and mitochondrial DNA copy number (mtDNA-CN) could serve as a biomarker for the adenoma or CRC formation. TL, mtDNA-CN, telomerase reverse transcriptase (TERT), and mitochondrial transcription factor A (TFAM) expressions were studied in 132 adenoma and 95 early-stage CRC patients. TL and mtDNA-CN were measured by multiplex quantitative polymerase chain reaction (qPCR). Expression of TERT and TFAM was measured by reverse transcription-qPCR. Significant TL shortening was observed in adenomas (P = 8.96e-14), tumor-node-metastasis (TNM) I (P = 3.49e-05), and TNM II (P = 2.29e-04) stages compared to the adjacent mucosa. This tendency was also contingent on TERT expression. Differential TFAM expression was observed in all groups, but an elevated relative mtDNA-CN was, compared to the adjacent mucosa, detected only in adenomas (P = 1.50e-08), where it correlated with TL (P = 4.10e-03). Notably, mtDNA-CN levels were significantly higher in adenomas than in early-stage tumors (TNM I, P = 2.00e-02; TNM II, P = 2.40e-02), suggesting a progressive decline during tumorigenesis. We have provided fresh insights into the crosstalk between telomere and mitochondrial biology in CRC precursors. These findings hold promise in understanding adenoma formation and CRC progression, as mtDNA-CN elevation and its association with TL were specific to precancerous lesions and were lost with progression to tumor.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30204 - Oncology

Project

—

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2026

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

MUTAGENESIS

ISSN

0267-8357

e-ISSN

1464-3804

Volume of the periodical

41

Issue of the periodical within the volume

3

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

142-155

UT code for WoS article

001716152100001

EID of the result in the Scopus database

2-s2.0-105036877938