Effects of redox conditions and zinc(II) ions on metallothionein aggregation revealed by chip capillary electrophoresis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F10%3A6387" target="_blank" >RIV/00064203:_____/10:6387 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/10:00040656 RIV/62156489:43210/10:00166516 RIV/00216208:11130/10:6387
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Effects of redox conditions and zinc(II) ions on metallothionein aggregation revealed by chip capillary electrophoresis
Original language description
We used capillary electrophoresis on chip coupled with fluorescence detection to determine structural changes of metallothionein (MT) with increasing concentration of zinc(II) ions and under various redox conditions. To investigate the structural-dependent effects, reduced and oxidized apo-MT was prepared. Zinc binding into reduced and oxidized apo-MT was compared. Formation of MT aggregates with increasing zinc concentration was observed by spectrophotometry, chip capillary electrophoresis, and SDS-PAGE. We found out that reduced MT forms aggregates more readily compared to oxidized MT. Using the chip capillary electrophoresis allowed us quantification of MT aggregation as a decrease in the area of the signal corresponding to the monomer form of MT and its ratio to total signal. The dependences had an exponential character. Zn-MT interaction was 30% faster during the first 15 min and 50% faster during the whole experiment for reduced MT.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Chromatography A
ISSN
0021-9673
e-ISSN
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Volume of the periodical
1217
Issue of the periodical within the volume
51
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
6
Pages from-to
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UT code for WoS article
000285429700006
EID of the result in the Scopus database
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