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Effects of redox conditions and zinc(II) ions on metallothionein aggregation revealed by chip capillary electrophoresis

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F10%3A6387" target="_blank" >RIV/00064203:_____/10:6387 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216224:14110/10:00040656 RIV/62156489:43210/10:00166516 RIV/00216208:11130/10:6387

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Effects of redox conditions and zinc(II) ions on metallothionein aggregation revealed by chip capillary electrophoresis

  • Original language description

    We used capillary electrophoresis on chip coupled with fluorescence detection to determine structural changes of metallothionein (MT) with increasing concentration of zinc(II) ions and under various redox conditions. To investigate the structural-dependent effects, reduced and oxidized apo-MT was prepared. Zinc binding into reduced and oxidized apo-MT was compared. Formation of MT aggregates with increasing zinc concentration was observed by spectrophotometry, chip capillary electrophoresis, and SDS-PAGE. We found out that reduced MT forms aggregates more readily compared to oxidized MT. Using the chip capillary electrophoresis allowed us quantification of MT aggregation as a decrease in the area of the signal corresponding to the monomer form of MT and its ratio to total signal. The dependences had an exponential character. Zn-MT interaction was 30% faster during the first 15 min and 50% faster during the whole experiment for reduced MT.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2010

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Chromatography A

  • ISSN

    0021-9673

  • e-ISSN

  • Volume of the periodical

    1217

  • Issue of the periodical within the volume

    51

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    6

  • Pages from-to

  • UT code for WoS article

    000285429700006

  • EID of the result in the Scopus database