Study of zinc-dependent aggregation of metallothionein from human prostatic cancer cell lines
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F10%3A00184955" target="_blank" >RIV/62156489:43210/10:00184955 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Study of zinc-dependent aggregation of metallothionein from human prostatic cancer cell lines
Original language description
Background: Metallothioneins (MTs) are heavy-metal binding cysteine-rich proteins. MTs are thought to play a role in tumour disease. They can be used for studying of course, prognosis and metastating of a tumour. The enhanced MTs expression leads to theformation of cytostatics resistance in tumour cells. One the current accepted opinion is that these mechanisms are closely connected to ability of MT to bind heavy metal ions and form aggregates. The aim of this work was to study the changes in aggregation of MT under various concentrations of zinc and different redox conditions in human prostatic cell lines. Material and Methods: Reduced apo-MT was oxidized with 0.75 and 3.5 % H2O2, 0.5 and 1 mMK2Cr2O7, and 0.5 and 1 mM KMnO4 for 2 hours at 37C. To thereduced and oxidized apo-MT ZnCl2 was added. Reaction was measured spectrometrically for 1 hour at 37 C. MT oxidation and interaction with zinc was evaluated by measuring of spectra within the range from 200 to 300 nm. Human cancer prost
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
—
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
European Journal of Cancer
ISSN
0959-8049
e-ISSN
—
Volume of the periodical
8
Issue of the periodical within the volume
5
Country of publishing house
GB - UNITED KINGDOM
Number of pages
1
Pages from-to
49
UT code for WoS article
—
EID of the result in the Scopus database
—