Chip gel electrophoresis as a tool for study of matrix metalloproteinase 9 interaction with metallothionein

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F11%3A7594" target="_blank" >RIV/00064203:_____/11:7594 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43210/11:00173509 RIV/00216305:26220/11:PU94853 RIV/00216208:11130/11:7594

Result on the web

<a href="http://dx.doi.org/10.1002/elps.201000526" target="_blank" >http://dx.doi.org/10.1002/elps.201000526</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Chip gel electrophoresis as a tool for study of matrix metalloproteinase 9 interaction with metallothionein

Original language description

Matrix metalloproteinases (MMPs) play numerous roles in physiological and pathological processes including cancer. Interactions of MMPs with other zinc-binding proteins are of great interest mainly from the point of view of the participation of these proteins in carcinogenesis. The aim of this study was to utilize chip gel electrophoresis for investigation of matrix metalloproteinase 9 (gelatinase B, MMP-9) interactions with collagen. The interaction was observed and the effect of temperature was tested. We further focused on the study of activation of MMP-9 by the presence of zinc-binding proteins called metallothioneins (MT). We confirmed the basic presumption of the activation of MMP-9 by the presence of MT similar to the temperature effect. Moreover, we chose the method of differential pulse voltammetry Brdicka reaction to verify our results. It can be concluded that the activity of MMP-9 was higher in the presence of MT in comparison with the experimental collagen(type I)-MMP-9 mi

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electrophoresis

ISSN

0173-0835

e-ISSN

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Volume of the periodical

32

Issue of the periodical within the volume

8

Country of publishing house

DE - GERMANY

Number of pages

4

Pages from-to

857-860

UT code for WoS article

000289352900009

EID of the result in the Scopus database

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