Real-time PCR quantification of major Wilms' tumor gene 1 (WT1) isoforms in acute myeloid leukemia, their characteristic expression patterns and possible functional consequences

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F12%3A8238" target="_blank" >RIV/00064203:_____/12:8238 - isvavai.cz</a>
Alternative codes found
RIV/00064190:_____/12:#0000369 RIV/00023736:_____/12:00009617 RIV/00216208:11130/12:8238
Result on the web
<a href="http://dx.doi.org/10.1038/leu.2012.76" target="_blank" >http://dx.doi.org/10.1038/leu.2012.76</a>
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
Real-time PCR quantification of major Wilms' tumor gene 1 (WT1) isoforms in acute myeloid leukemia, their characteristic expression patterns and possible functional consequences
Original language description
Wilms' tumor gene 1 (WT1) functions including some contradictory effects may be explained by the presence and interactions of its isoforms, however, their evaluation has been so far complicated by several technical problems. We designed unique quantitative PCR systems for direct quantification of the major WT1 isoforms A[EX5 - /KTS -], B[+ / -], C[- / +] and D[+ / +] and verified their sensitivity, specificity and reproducibility in extensive testing. With this method we evaluated WT1 total and isoformexpression in 23 normal bone marrow (BM) samples, 73 childhood acute myeloid leukemia (AML), 20 childhood myelodysplastic syndrome (MDS), 9 childhood severe aplastic anemia (SAA), 30 adult AML and 29 adult MDS patients. WT1 isoform patterns showed differences among these samples and clustered them into groups representing the specific diagnoses (P<0.0001). Isoform profiles were independent of total WT1 expression and possess certain common features-overexpression of isoform D and EX5[+]
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FD - Oncology and haematology
OECD FORD branch
—

Project
<a href="/en/project/NS10488" target="_blank" >NS10488: Expression pattern of WT1 gene isoforms and mutational status as a new pathogenetic and diagnostic marker in paediatric haematological malignancies</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)