Genetic and functional analyses do not explain the association of high PRC1 expression with poor survival of breast carcinoma patients

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F16%3A10324650" target="_blank" >RIV/00064203:_____/16:10324650 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11130/16:10324650 RIV/00216208:11140/16:10324650 RIV/00216208:11120/16:43912166 RIV/61989592:15110/16:33160452 RIV/75010330:_____/16:00011392

Result on the web

<a href="http://www.sciencedirect.com/science/article/pii/S0753332216308368" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0753332216308368</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.biopha.2016.07.047" target="_blank" >10.1016/j.biopha.2016.07.047</a>

Result language

angličtina

Original language name

Genetic and functional analyses do not explain the association of high PRC1 expression with poor survival of breast carcinoma patients

Original language description

Microtubules are vitally important for eukaryotic cell division. Therefore, we evaluated the relevance of mitotic kinesin KIF14, protein-regulating cytokinesis 1 (PRC1), and citron kinase (CIT) for the prognosis of breast carcinoma patients. Transcript levels were assessed by quantitative real-time PCR in tissues from two independent groups of breast carcinoma patients and compared with clinical data. Tissue PRC1 protein levels were estimated using immunoblotting, and the PRC1 tagged haplotype was analyzed in genomic DNA. A functional study was performed in MDA-MB-231 cells in vitro. KIF14, PRC1, and CIT transcripts were overexpressed in tumors compared with control tissues. Tumors without expression of hormonal receptors or high-grade tumors expressed significantly higher KIF14 and PRC1 levels than hormonally-positive or low-grade tumors. Patients with high intra-tumoral PRC1 levels had significantly worse disease-free survival than patients with low levels. PRC1 rs10520699 and rs11852999 polymorphisms were associated with PRC1 transcript levels, but not with patients' survival. Paclitaxel induced PRC1 expression, but PRC1 knockdown did not modify the paclitaxel cytotoxicity in vitro. PRC1 overexpression predicts poor disease-free survival of patients with breast carcinomas. Genetic variability of PRC1 and the protein interaction with paclitaxel cytotoxicity do not explain this association.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomedicine and Pharmacotherapy

ISSN

0753-3322

e-ISSN

—

Volume of the periodical

83

Issue of the periodical within the volume

October

Country of publishing house

FR - FRANCE

Number of pages

8

Pages from-to

857-864

UT code for WoS article

000390433400104

EID of the result in the Scopus database

2-s2.0-84980454176