Nanobiosensor for Rapid Detection of Creatinine Crystals Present in Urine

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F25%3A10503395" target="_blank" >RIV/00064203:_____/25:10503395 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11130/25:10503395
Result on the web
<a href="https://doi.org/10.37904/nanocon.2024.5023" target="_blank" >https://doi.org/10.37904/nanocon.2024.5023</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.37904/nanocon.2024.5023" target="_blank" >10.37904/nanocon.2024.5023</a>

Result language
angličtina
Original language name
Nanobiosensor for Rapid Detection of Creatinine Crystals Present in Urine
Original language description
Creatine is synthesized primarily in the liver and kidneys from amino acids and is phosphorylated to phosphocreatine, which serves as an energy source in muscle. Creatine is non-enzymatically degraded to creatinine at a constant rate and excreted in the urine. Its formation mainly depends on the muscle mass. Changes in creatine and creatinine levels may be related to various diseases (e.g. kidney disease, rhabdomyolysis etc.). Several methods are used to determine creatinine, such as for example the Jaffe method using the reaction of creatinine with picric acid in an alkaline medium, forming a red-orange complex or the enzymatic Trinder reaction. In some cases, crystals or larger aggregates are present in the urine. Primary diagnosis of such crystals is focused on microscopic evidence of the presence of the structure and may be confirmed by FTIR. The three enzymes, creatinase, creatininase and sarcosine oxidase can also be used for qualitative assessment of creatinine. The aim of this work was to design a SPION nanobiosensor for qualitative proof of the presence of creatinine in a sample. The methodology uses the highly sensitive Red Amplex fluorophore. Absorbance dependence on creatinine concentration read off at the wavelength of 572 nm was linear over a total range of 0 -1000 μM.
Czech name
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Czech description
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Nanocon Conference Proceedings International Conference on Nanomaterials
ISBN
978-80-88365-24-2
ISSN
2694-930X
e-ISSN
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Number of pages
8
Pages from-to
238-245
Publisher name
Tanger
Place of publication
Ostrava
Event location
Brno
Event date
Oct 16, 2024
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
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