Gender-related differences observed among immune cells in synovial fluid in knee osteoarthritis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00098892%3A_____%2F18%3AN0000029" target="_blank" >RIV/00098892:_____/18:N0000029 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15110/18:73587877 RIV/61989100:27240/18:10241688

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Gender-related differences observed among immune cells in synovial fluid in knee osteoarthritis

Original language description

Gender-related differences observed among immune cells in synovial fluid in knee osteoarthritis Anotace anglicky OBJECTIVE: There is no existing comprehensive report on the cellular composition of synovial fluids (SFs) from knee osteoarthritis (OA). We therefore aimed to characterise the immune cell composition in SFs from knee OA (KOA) and in subgroups according to gender. DESIGN: The immunophenotyping of monocyte/macrophage lineage cells, T and B cells, NK cells, neutrophils, dendritic and mast cells (MC) present in SFs from 53 patients (24 males/29 females) with KOA was performed using 6-colour flow cytometry. RESULTS: SFs from patients with OA contained 90 % hematopoietic cells. Lymphocytes were the predominant cell population (44.8 %) in the SFs of OA patients, with CD4+ T lymphocytes being more prevalent than CD8+ T cells (CD4+/CD8+ ratio = 1.3). Within the monocyte/macrophage lineage gating, monocytes accounted for 33.9 %, macrophages 14.8 %, myeloid dendritic cells 16.4 %. The rest of the hematopoietic cells were comprised of neutrophils (8 %), NK cells (3.8 %), T regulatory cells (1.2 %), plasmacytoid dendritic cells (1.1 %), mast cells (0.3 %). In OA females, a higher percentage of CD4+ T cells (P = 0.023), macrophages (P = 0.012), and a lower percentage of monocytes (P = 0.008) and CD8+ T cells (P = 0.002) were detected in comparison to OA males. CONCLUSIONS: Based on the immune cell composition of SFs, data mining analysis revealed distinct phenotypes (monocyte- and lymphocyte-predominant) within each gender group. This first study on the cellular complexity of SFs in KOA showed marked differences between male and female patients. The findings give a rational starting point for patient stratification according to their phenotypes, as is required for phenotype-specific treatment strategies.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30211 - Orthopaedics

Project

<a href="/en/project/NV16-31852A" target="_blank" >NV16-31852A: Prediction for reoperation in patients with THA and TKA based on immunogenetic signature: development of risk calculator for routine clinical practice</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Osteoarthritis and Cartilage

ISSN

1063-4584

e-ISSN

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Volume of the periodical

26

Issue of the periodical within the volume

9

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

1247-1256

UT code for WoS article

000441526000013

EID of the result in the Scopus database

2-s2.0-85047333270